TY - JOUR
T1 - An antisense transcript to SMAD5 expressed in fetal and tumor tissues
AU - Zavadil, Jiri
AU - Svoboda, Petr
AU - Liang, Hong
AU - Kottickal, Lazar V.
AU - Nagarajan, Lalitha
N1 - Funding Information:
We thank Shourong Zhao for the β-2-microglobulin primers and Dr. Yunfang Jiang and Wei Guo for helpful discussion. This study was supported by NIH Grants CA66982 and CA55164 and by the Internal Grant Agency of the Ministry of Health, Czech Republic, Grants 2826-3 and NC/5535/3.
PY - 1999/2/24
Y1 - 1999/2/24
N2 - SMAD5, a transducer of TGF-β/BMP inhibitory signals and a tumor suppressor candidate, localizes to the region of invariant loss in human myeloid neoplasms, on chromosome 5q31.1. Recent evidence indicates a gene-dosage effect along the TGF-β/BMP signaling pathways. We have identified a novel transcript designated DAMS, whose 3' exonic sequences contain in part an alternate 5' exon of SMAD5, in the antisense orientation. Expressed sequenced tags (ESTs) for DAMS are found in fetal tissues (heart, adrenal glands, and total fetus) and pancreatic tumor cDNA libraries. In contrast to SMAD5, DAMS expression is not readily detectable in adult and fetal tissues. Semiquantitative PCR suggests that the stoichiometry between SMAD5 and DAMS transcripts ranges between 15 and 120 in normal and malignant hematopoietic cells. The findings raise the possibility that DAMS may be a fail-safe mechanism for precise regulation of SMAD5 transcript levels that may be critical in maintaining normal homeostasis.
AB - SMAD5, a transducer of TGF-β/BMP inhibitory signals and a tumor suppressor candidate, localizes to the region of invariant loss in human myeloid neoplasms, on chromosome 5q31.1. Recent evidence indicates a gene-dosage effect along the TGF-β/BMP signaling pathways. We have identified a novel transcript designated DAMS, whose 3' exonic sequences contain in part an alternate 5' exon of SMAD5, in the antisense orientation. Expressed sequenced tags (ESTs) for DAMS are found in fetal tissues (heart, adrenal glands, and total fetus) and pancreatic tumor cDNA libraries. In contrast to SMAD5, DAMS expression is not readily detectable in adult and fetal tissues. Semiquantitative PCR suggests that the stoichiometry between SMAD5 and DAMS transcripts ranges between 15 and 120 in normal and malignant hematopoietic cells. The findings raise the possibility that DAMS may be a fail-safe mechanism for precise regulation of SMAD5 transcript levels that may be critical in maintaining normal homeostasis.
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U2 - 10.1006/bbrc.1999.0256
DO - 10.1006/bbrc.1999.0256
M3 - Article
C2 - 10049768
AN - SCOPUS:0033599323
SN - 0006-291X
VL - 255
SP - 668
EP - 672
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 3
ER -