An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein

Helen L. Fillmore; John Shurm; Peggy Furqueron; Sujit S. Prabhu; George T. Gillies; William C. Broaddus

doi:10.1016/S0304-3835(99)00053-1

An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein

Helen L. Fillmore, John Shurm, Peggy Furqueron, Sujit S. Prabhu, George T. Gillies, William C. Broaddus

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

In our studies examining mechanisms of brain tumor cell migration/invasion into host tissue, the RT2 rat glioma cell line was stably transfected utilizing a green fluorescent protein (GFP) DNA construct. Stable transfected RT2 cells demonstrate high GFP expression at initial passages, maintain expression over 30 passages and have similar in vitro and in vivo growth patterns relative to controls. In rat brain tissue, individual tumor cells can be detected without any post-treatment. Using flow cytometry and cell sorting, we are able to retrieve and culture GFP positive cells from tumor core as well as adjacent tissue and contralateral hemisphere of tumor-bearing animals. Copyright (C) 1999.

Original language	English (US)
Pages (from-to)	9-19
Number of pages	11
Journal	Cancer Letters
Volume	141
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(99)00053-1
State	Published - Jul 1 1999
Externally published	Yes

Keywords

Brain tumor
GBM
Green fluorescent protein (GFP)
In vivo
Invasion
Metastasis

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/S0304-3835(99)00053-1

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title = "An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein",

abstract = "In our studies examining mechanisms of brain tumor cell migration/invasion into host tissue, the RT2 rat glioma cell line was stably transfected utilizing a green fluorescent protein (GFP) DNA construct. Stable transfected RT2 cells demonstrate high GFP expression at initial passages, maintain expression over 30 passages and have similar in vitro and in vivo growth patterns relative to controls. In rat brain tissue, individual tumor cells can be detected without any post-treatment. Using flow cytometry and cell sorting, we are able to retrieve and culture GFP positive cells from tumor core as well as adjacent tissue and contralateral hemisphere of tumor-bearing animals. Copyright (C) 1999.",

keywords = "Brain tumor, GBM, Green fluorescent protein (GFP), In vivo, Invasion, Metastasis",

author = "Fillmore, {Helen L.} and John Shurm and Peggy Furqueron and Prabhu, {Sujit S.} and Gillies, {George T.} and Broaddus, {William C.}",

note = "Funding Information: This study was supported by the Lind Lawrence Foundation and the MCV Brain Tumor Research and Support Funds. We are especially grateful to the Crone, Cullather and Hord families for their support. The authors would like to thank Drs. Harold Young, Barbara Amaker, Martin Graf and Timothy Van Meter for helpful discussions and critical review of this manuscript and to Fay Akers for her excellent editorial assistance. We thank also Dr. Patricia Carlson and Francis White for their help with flow cytometry experiments. Aided by grants IN-105 from the American Cancer Society (HF) and NS01766 from the National Institutes of Health (WCB).",

year = "1999",

month = jul,

day = "1",

doi = "10.1016/S0304-3835(99)00053-1",

language = "English (US)",

volume = "141",

pages = "9--19",

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T1 - An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein

AU - Fillmore, Helen L.

AU - Shurm, John

AU - Furqueron, Peggy

AU - Prabhu, Sujit S.

AU - Gillies, George T.

AU - Broaddus, William C.

N1 - Funding Information: This study was supported by the Lind Lawrence Foundation and the MCV Brain Tumor Research and Support Funds. We are especially grateful to the Crone, Cullather and Hord families for their support. The authors would like to thank Drs. Harold Young, Barbara Amaker, Martin Graf and Timothy Van Meter for helpful discussions and critical review of this manuscript and to Fay Akers for her excellent editorial assistance. We thank also Dr. Patricia Carlson and Francis White for their help with flow cytometry experiments. Aided by grants IN-105 from the American Cancer Society (HF) and NS01766 from the National Institutes of Health (WCB).

PY - 1999/7/1

Y1 - 1999/7/1

N2 - In our studies examining mechanisms of brain tumor cell migration/invasion into host tissue, the RT2 rat glioma cell line was stably transfected utilizing a green fluorescent protein (GFP) DNA construct. Stable transfected RT2 cells demonstrate high GFP expression at initial passages, maintain expression over 30 passages and have similar in vitro and in vivo growth patterns relative to controls. In rat brain tissue, individual tumor cells can be detected without any post-treatment. Using flow cytometry and cell sorting, we are able to retrieve and culture GFP positive cells from tumor core as well as adjacent tissue and contralateral hemisphere of tumor-bearing animals. Copyright (C) 1999.

AB - In our studies examining mechanisms of brain tumor cell migration/invasion into host tissue, the RT2 rat glioma cell line was stably transfected utilizing a green fluorescent protein (GFP) DNA construct. Stable transfected RT2 cells demonstrate high GFP expression at initial passages, maintain expression over 30 passages and have similar in vitro and in vivo growth patterns relative to controls. In rat brain tissue, individual tumor cells can be detected without any post-treatment. Using flow cytometry and cell sorting, we are able to retrieve and culture GFP positive cells from tumor core as well as adjacent tissue and contralateral hemisphere of tumor-bearing animals. Copyright (C) 1999.

KW - Brain tumor

KW - GBM

KW - Green fluorescent protein (GFP)

KW - In vivo

KW - Invasion

KW - Metastasis

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AN - SCOPUS:0032979524

SN - 0304-3835

VL - 141

SP - 9

EP - 19

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein

Abstract

Keywords

ASJC Scopus subject areas

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