An inducible expression system for the production of human lactoferrin in Aspergillus nidulans

Pauline P. Ward; Gregory S. May; Denis R. Headon; Orla M. Conneely

doi:10.1016/0378-1119(92)90054-S

An inducible expression system for the production of human lactoferrin in Aspergillus nidulans

Pauline P. Ward, Gregory S. May, Denis R. Headon, Orla M. Conneely

Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.

Original language	English (US)
Pages (from-to)	219-223
Number of pages	5
Journal	Gene
Volume	122
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90054-S
State	Published - Dec 1 1992

Keywords

Recombinant DNA
alcA promoter
filamentous fungi
functional assay
iron-binding glycoprotein
secretion

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(92)90054-S

Cite this

@article{31a38b1c24504628b3af92071ff40c37,

title = "An inducible expression system for the production of human lactoferrin in Aspergillus nidulans",

abstract = "The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.",

keywords = "Recombinant DNA, alcA promoter, filamentous fungi, functional assay, iron-binding glycoprotein, secretion",

author = "Ward, {Pauline P.} and May, {Gregory S.} and Headon, {Denis R.} and Conneely, {Orla M.}",

year = "1992",

month = dec,

day = "1",

doi = "10.1016/0378-1119(92)90054-S",

language = "English (US)",

volume = "122",

pages = "219--223",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - An inducible expression system for the production of human lactoferrin in Aspergillus nidulans

AU - Ward, Pauline P.

AU - May, Gregory S.

AU - Headon, Denis R.

AU - Conneely, Orla M.

PY - 1992/12/1

Y1 - 1992/12/1

N2 - The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.

AB - The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (rehLF) is produced at levels up to 5 μg/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.

KW - Recombinant DNA

KW - alcA promoter

KW - filamentous fungi

KW - functional assay

KW - iron-binding glycoprotein

KW - secretion

UR - http://www.scopus.com/inward/record.url?scp=0026448560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026448560&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(92)90054-S

DO - 10.1016/0378-1119(92)90054-S

M3 - Article

C2 - 1452033

AN - SCOPUS:0026448560

SN - 0378-1119

VL - 122

SP - 219

EP - 223

JO - Gene

JF - Gene

IS - 1

ER -

An inducible expression system for the production of human lactoferrin in Aspergillus nidulans

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this