An integrated transcript map of human chromosome 1p35-p36

Sanford J. Jensen; Erik P. Sulman; John M. Maris; Tara C. Matise; Patrick J. Vojta; J. Carl Barrett; Garrett M. Brodeur; Peter S. White

doi:10.1006/geno.1997.4714

An integrated transcript map of human chromosome 1p35-p36

Sanford J. Jensen, Erik P. Sulman, John M. Maris, Tara C. Matise, Patrick J. Vojta, J. Carl Barrett, Garrett M. Brodeur, Peter S. White

Research output: Contribution to journal › Article › peer-review

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Abstract

The distal short arm of human chromosome 1 (1p) is rearranged in a variety of malignancies, and several genetic diseases also map to this region. We have constructed an integrated transcript map to precisely define the positions of genes and expressed sequence tags (ESTs) previously mapped to 1p35-p36, a region spanning approximately 40 Mb. To anchor the integrated map, a framework genetic map was constructed with 24 genetic markers and a marker order of >1000:1 odds, yielding an average resolution of 2.8 cM. An additional 106 genetic markers were localized relative to the framework genetic map. To place markers more precisely within 1p35-p36, a chromosome 1- specific, radiation-reduced hybrid (RH) panel was created. Individual DNA fragments of the RH panel were identified and ordered by PCR with the framework genetic map. A total of 250 markers, including 142 genes and ESTs, were mapped by PCR against the RH panel. The map has an observed resolution of 800 kb, and the results closely match and more precisely define previous mapping information for most markers. This map will help to identify candidate genes for genetic diseases mapping to distal 1p and is fully integrated with existing genetic and RH maps of the human genome.

Original language	English (US)
Pages (from-to)	126-136
Number of pages	11
Journal	Genomics
Volume	42
Issue number	1
DOIs	https://doi.org/10.1006/geno.1997.4714
State	Published - May 15 1997

ASJC Scopus subject areas

Genetics

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10.1006/geno.1997.4714

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@article{6d002e09bca04301b834425f425582ef,

title = "An integrated transcript map of human chromosome 1p35-p36",

abstract = "The distal short arm of human chromosome 1 (1p) is rearranged in a variety of malignancies, and several genetic diseases also map to this region. We have constructed an integrated transcript map to precisely define the positions of genes and expressed sequence tags (ESTs) previously mapped to 1p35-p36, a region spanning approximately 40 Mb. To anchor the integrated map, a framework genetic map was constructed with 24 genetic markers and a marker order of >1000:1 odds, yielding an average resolution of 2.8 cM. An additional 106 genetic markers were localized relative to the framework genetic map. To place markers more precisely within 1p35-p36, a chromosome 1- specific, radiation-reduced hybrid (RH) panel was created. Individual DNA fragments of the RH panel were identified and ordered by PCR with the framework genetic map. A total of 250 markers, including 142 genes and ESTs, were mapped by PCR against the RH panel. The map has an observed resolution of 800 kb, and the results closely match and more precisely define previous mapping information for most markers. This map will help to identify candidate genes for genetic diseases mapping to distal 1p and is fully integrated with existing genetic and RH maps of the human genome.",

author = "Jensen, {Sanford J.} and Sulman, {Erik P.} and Maris, {John M.} and Matise, {Tara C.} and Vojta, {Patrick J.} and Barrett, {J. Carl} and Brodeur, {Garrett M.} and White, {Peter S.}",

note = "Funding Information: The authors gratefully acknowledge K. Arden, A. Ashkenazi, R. Conaway, H. Donis-Keller, N. Dracopoli, C. Helms, N. Ikegaki, G. Landes, Y. Nakamura, and A. Weith for clones and/or primer sequences; the Children's Hospital of Philadelphia nucleic acid/protein research core facility for DNA sequencing; and S. Kyemba for technical assistance. This work was supported in part by Grants CA39771 (G.M.B.) and HG00008 (T.C.M.) from the National Institutes of Health.",

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AU - Jensen, Sanford J.

AU - Sulman, Erik P.

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AU - Matise, Tara C.

AU - Vojta, Patrick J.

AU - Barrett, J. Carl

AU - Brodeur, Garrett M.

AU - White, Peter S.

N1 - Funding Information: The authors gratefully acknowledge K. Arden, A. Ashkenazi, R. Conaway, H. Donis-Keller, N. Dracopoli, C. Helms, N. Ikegaki, G. Landes, Y. Nakamura, and A. Weith for clones and/or primer sequences; the Children's Hospital of Philadelphia nucleic acid/protein research core facility for DNA sequencing; and S. Kyemba for technical assistance. This work was supported in part by Grants CA39771 (G.M.B.) and HG00008 (T.C.M.) from the National Institutes of Health.

PY - 1997/5/15

Y1 - 1997/5/15

N2 - The distal short arm of human chromosome 1 (1p) is rearranged in a variety of malignancies, and several genetic diseases also map to this region. We have constructed an integrated transcript map to precisely define the positions of genes and expressed sequence tags (ESTs) previously mapped to 1p35-p36, a region spanning approximately 40 Mb. To anchor the integrated map, a framework genetic map was constructed with 24 genetic markers and a marker order of >1000:1 odds, yielding an average resolution of 2.8 cM. An additional 106 genetic markers were localized relative to the framework genetic map. To place markers more precisely within 1p35-p36, a chromosome 1- specific, radiation-reduced hybrid (RH) panel was created. Individual DNA fragments of the RH panel were identified and ordered by PCR with the framework genetic map. A total of 250 markers, including 142 genes and ESTs, were mapped by PCR against the RH panel. The map has an observed resolution of 800 kb, and the results closely match and more precisely define previous mapping information for most markers. This map will help to identify candidate genes for genetic diseases mapping to distal 1p and is fully integrated with existing genetic and RH maps of the human genome.

AB - The distal short arm of human chromosome 1 (1p) is rearranged in a variety of malignancies, and several genetic diseases also map to this region. We have constructed an integrated transcript map to precisely define the positions of genes and expressed sequence tags (ESTs) previously mapped to 1p35-p36, a region spanning approximately 40 Mb. To anchor the integrated map, a framework genetic map was constructed with 24 genetic markers and a marker order of >1000:1 odds, yielding an average resolution of 2.8 cM. An additional 106 genetic markers were localized relative to the framework genetic map. To place markers more precisely within 1p35-p36, a chromosome 1- specific, radiation-reduced hybrid (RH) panel was created. Individual DNA fragments of the RH panel were identified and ordered by PCR with the framework genetic map. A total of 250 markers, including 142 genes and ESTs, were mapped by PCR against the RH panel. The map has an observed resolution of 800 kb, and the results closely match and more precisely define previous mapping information for most markers. This map will help to identify candidate genes for genetic diseases mapping to distal 1p and is fully integrated with existing genetic and RH maps of the human genome.

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An integrated transcript map of human chromosome 1p35-p36

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