An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity

A. M. Killary; R. E.K. Fournier

doi:10.1016/0014-4827(84)90168-X

An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity

A. M. Killary, R. E.K. Fournier

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Abstract

A selective system toxic towards mammalian cells expressing the liver-specific isozyme of alcohol dehydrogenase (L-ADH) has been developed. A number of α-unsaturated primary and secondary alcohols were assayed for their ability to serve as substates for rat liver ADH and were screened for cytotoxicity towards L-ADH⁺ and L-ADH^- cells. 1-Propen-3-ol and 1-penten-3-ol were identified as agents showing selective cytotoxicity. Reconstruction experiments demonstrated that 1-propen-3-ol at a concentration of 15 μM could be used to recover L-ADH^- clones from mixed populations of L-ADH⁺ and LADH^- cells. Cells expressing the non-allelic S-ADH isozyme were not killed under these conditions. The selective system defined in this report is thus isozyme-specific.

Original language	English (US)
Pages (from-to)	442-453
Number of pages	12
Journal	Experimental Cell Research
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(84)90168-X
State	Published - Oct 1984
Externally published	Yes

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Cell Biology

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10.1016/0014-4827(84)90168-X

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@article{08199e74f44e44c2b069c7fbb2c78b02,

title = "An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity",

abstract = "A selective system toxic towards mammalian cells expressing the liver-specific isozyme of alcohol dehydrogenase (L-ADH) has been developed. A number of α-unsaturated primary and secondary alcohols were assayed for their ability to serve as substates for rat liver ADH and were screened for cytotoxicity towards L-ADH+ and L-ADH- cells. 1-Propen-3-ol and 1-penten-3-ol were identified as agents showing selective cytotoxicity. Reconstruction experiments demonstrated that 1-propen-3-ol at a concentration of 15 μM could be used to recover L-ADH- clones from mixed populations of L-ADH+ and LADH- cells. Cells expressing the non-allelic S-ADH isozyme were not killed under these conditions. The selective system defined in this report is thus isozyme-specific.",

author = "Killary, {A. M.} and Fournier, {R. E.K.}",

note = "Funding Information: The valuable technical assistanceo f F. R. Parker, M. M. Smith, and S. F. Waelder is acknowledged. These studies were supported by grant GM26449 from NIH. A. M. K. was supported by postdoctoralf ellowshipsfrom the American Cancer Society, California Division, and the NIH. R. E. K. F. is the recipient of an American Cancer Society Faculty Research Award.",

year = "1984",

month = oct,

doi = "10.1016/0014-4827(84)90168-X",

language = "English (US)",

volume = "154",

pages = "442--453",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity

AU - Killary, A. M.

AU - Fournier, R. E.K.

N1 - Funding Information: The valuable technical assistanceo f F. R. Parker, M. M. Smith, and S. F. Waelder is acknowledged. These studies were supported by grant GM26449 from NIH. A. M. K. was supported by postdoctoralf ellowshipsfrom the American Cancer Society, California Division, and the NIH. R. E. K. F. is the recipient of an American Cancer Society Faculty Research Award.

PY - 1984/10

Y1 - 1984/10

N2 - A selective system toxic towards mammalian cells expressing the liver-specific isozyme of alcohol dehydrogenase (L-ADH) has been developed. A number of α-unsaturated primary and secondary alcohols were assayed for their ability to serve as substates for rat liver ADH and were screened for cytotoxicity towards L-ADH+ and L-ADH- cells. 1-Propen-3-ol and 1-penten-3-ol were identified as agents showing selective cytotoxicity. Reconstruction experiments demonstrated that 1-propen-3-ol at a concentration of 15 μM could be used to recover L-ADH- clones from mixed populations of L-ADH+ and LADH- cells. Cells expressing the non-allelic S-ADH isozyme were not killed under these conditions. The selective system defined in this report is thus isozyme-specific.

AB - A selective system toxic towards mammalian cells expressing the liver-specific isozyme of alcohol dehydrogenase (L-ADH) has been developed. A number of α-unsaturated primary and secondary alcohols were assayed for their ability to serve as substates for rat liver ADH and were screened for cytotoxicity towards L-ADH+ and L-ADH- cells. 1-Propen-3-ol and 1-penten-3-ol were identified as agents showing selective cytotoxicity. Reconstruction experiments demonstrated that 1-propen-3-ol at a concentration of 15 μM could be used to recover L-ADH- clones from mixed populations of L-ADH+ and LADH- cells. Cells expressing the non-allelic S-ADH isozyme were not killed under these conditions. The selective system defined in this report is thus isozyme-specific.

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U2 - 10.1016/0014-4827(84)90168-X

DO - 10.1016/0014-4827(84)90168-X

M3 - Article

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AN - SCOPUS:0021203908

SN - 0014-4827

VL - 154

SP - 442

EP - 453

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity

Abstract

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