TY - JOUR
T1 - An orthotopic model of papillary thyroid carcinoma in athymic nude mice
AU - Ahn, Soon Hyun
AU - Henderson, Ying
AU - Kang, Ya'an
AU - Chattopadhyay, Chandrani
AU - Holton, Paula
AU - Wang, Mary
AU - Briggs, Katrina
AU - Clayman, Gary L.
PY - 2008/2
Y1 - 2008/2
N2 - Objective: To develop a reproducible orthotopic model of papillary thyroid carcinoma for the BRAFV600E mutation (GenBank NM004333) and an RET/PTC rearrangement (GenBank M31213) that recapitulates the clinical picture in humans. Design: In vitro and in vivo study. Setting: Department of Head and Neck Surgery, M. D. Anderson Cancer Center. Subjects: Eight- to 12-week-old athymic female nude mice. Interventions: Either BRAF-mutated or RET/PTC1-rearranged papillary thyroid carcinoma cells were injected into the thyroid glands of athymic female nude mice. The mice were euthanized when the tumor burden exceeded 1.0 cm or when they exhibited significant morbidity. Main Outcome Measures: Tumorigenicity, extent of tumor invasion and metastasis, cell invasion and migration, and median survival. Results: All the BRAF-mutated cell lines and 1 selected RET/PTC1-rearranged cell line were 100% tumorigenic in mice. These mouse tumor models exhibited a wide range of biological potential, including laryngeal invasion, lymph node metastasis, and pulmonary metastasis, thus reflecting the clinical spectrum of papillary carcinoma. Conclusions: An orthotopic model of papillary thyroid carcinoma was successfully established in nude mice using BRAF-mutated and RET/PTC1-rearranged cell lines. These models mimic the human disease and will thus be useful for evaluating the clinical potential of novel targeted therapies.
AB - Objective: To develop a reproducible orthotopic model of papillary thyroid carcinoma for the BRAFV600E mutation (GenBank NM004333) and an RET/PTC rearrangement (GenBank M31213) that recapitulates the clinical picture in humans. Design: In vitro and in vivo study. Setting: Department of Head and Neck Surgery, M. D. Anderson Cancer Center. Subjects: Eight- to 12-week-old athymic female nude mice. Interventions: Either BRAF-mutated or RET/PTC1-rearranged papillary thyroid carcinoma cells were injected into the thyroid glands of athymic female nude mice. The mice were euthanized when the tumor burden exceeded 1.0 cm or when they exhibited significant morbidity. Main Outcome Measures: Tumorigenicity, extent of tumor invasion and metastasis, cell invasion and migration, and median survival. Results: All the BRAF-mutated cell lines and 1 selected RET/PTC1-rearranged cell line were 100% tumorigenic in mice. These mouse tumor models exhibited a wide range of biological potential, including laryngeal invasion, lymph node metastasis, and pulmonary metastasis, thus reflecting the clinical spectrum of papillary carcinoma. Conclusions: An orthotopic model of papillary thyroid carcinoma was successfully established in nude mice using BRAF-mutated and RET/PTC1-rearranged cell lines. These models mimic the human disease and will thus be useful for evaluating the clinical potential of novel targeted therapies.
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U2 - 10.1001/archoto.2007.36
DO - 10.1001/archoto.2007.36
M3 - Article
C2 - 18283163
AN - SCOPUS:39649091396
SN - 0886-4470
VL - 134
SP - 190
EP - 197
JO - Archives of Otolaryngology - Head and Neck Surgery
JF - Archives of Otolaryngology - Head and Neck Surgery
IS - 2
ER -