Analysis of human blood memory T follicular helper subsets

Salah Eddine Bentebibel; Clement Jacquemin; Nathalie Schmitt; Hideki Ueno

doi:10.1007/978-1-4939-2498-1_16

Analysis of human blood memory T follicular helper subsets

Salah Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, Hideki Ueno

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Human blood contains a memory counterpart of T follicular helper (T_FH) cells. Blood T_FH cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory T_FH cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory T_FH subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory T_FH subsets.

Original language	English (US)
Pages (from-to)	187-197
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1291
DOIs	https://doi.org/10.1007/978-1-4939-2498-1_16
State	Published - 2015
Externally published	Yes

Keywords

Blood
CXCR5
Human
Subsets
T follicular helper cells

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2498-1_16

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title = "Analysis of human blood memory T follicular helper subsets",

abstract = "Human blood contains a memory counterpart of T follicular helper (TFH) cells. Blood TFH cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory TFH cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory TFH subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory TFH subsets.",

keywords = "Blood, CXCR5, Human, Subsets, T follicular helper cells",

author = "Bentebibel, {Salah Eddine} and Clement Jacquemin and Nathalie Schmitt and Hideki Ueno",

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doi = "10.1007/978-1-4939-2498-1_16",

language = "English (US)",

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AU - Bentebibel, Salah Eddine

AU - Jacquemin, Clement

AU - Schmitt, Nathalie

AU - Ueno, Hideki

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - Human blood contains a memory counterpart of T follicular helper (TFH) cells. Blood TFH cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory TFH cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory TFH subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory TFH subsets.

AB - Human blood contains a memory counterpart of T follicular helper (TFH) cells. Blood TFH cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory TFH cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory TFH subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory TFH subsets.

KW - Blood

KW - CXCR5

KW - Human

KW - Subsets

KW - T follicular helper cells

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JF - Methods in Molecular Biology

ER -

Analysis of human blood memory T follicular helper subsets

Abstract

Keywords

ASJC Scopus subject areas

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