TY - JOUR
T1 - Analysis of inhibitor of apoptosis protein family expression during mammary gland development
AU - Owens, Thomas W.
AU - Foster, Fiona M.
AU - Tanianis-Hughes, Jolanta
AU - Cheung, Julia Y.
AU - Brackenbury, Lisa
AU - Streuli, Charles H.
N1 - Funding Information:
This work was supported by funding from the Wellcome Trust and Breast Cancer Campaign. We thank John Silke (LaTrobe University, Melbourne, Australia) for generously supplying the anti-cIAP1 antibody. We would like to thank Dr Rebecca Rock for her help and advice with the quantitative PCR analysis and to Dr Giovanna Collu for her advice on the manuscript. Thanks also to Dr Andy Hayes and his team in the Faculty of Life Sciences Microarray Facility for their advice and use of equipment.
PY - 2010
Y1 - 2010
N2 - Background. Inhibitors-of-Apoptosis-Proteins (IAPs) are an evolutionarily conserved family of proteins capable of regulating several facets of apoptosis. IAPs are frequently dysregulated in cancer, but their role in the regulation of apoptosis during developmental processes is not fully understood. Here we examined the expression of IAPs during the post-natal development of the mouse mammary gland, which is a tissue that exhibits a profound induction of apoptosis during involution. Results. Six out of eight mammalian IAP family members are expressed in the mammary gland. Notably, quantitative PCR and immunoblotting revealed that XIAP, c-IAP1 and c-IAP2 are down-regulated in pregnancy and lactation, and prior to the onset of involution. In cultured mammary epithelial cells (MECs), XIAP levels decreased in response to inhibition of growth factor signalling. Maintaining XIAP levels in MECs by expressing exogenous XIAP protected them from all apoptotic stimuli tested. Conclusions. These data suggest that the developmental regulation of IAP expression in vivo contributes to naturally occurring programmes of cell death.
AB - Background. Inhibitors-of-Apoptosis-Proteins (IAPs) are an evolutionarily conserved family of proteins capable of regulating several facets of apoptosis. IAPs are frequently dysregulated in cancer, but their role in the regulation of apoptosis during developmental processes is not fully understood. Here we examined the expression of IAPs during the post-natal development of the mouse mammary gland, which is a tissue that exhibits a profound induction of apoptosis during involution. Results. Six out of eight mammalian IAP family members are expressed in the mammary gland. Notably, quantitative PCR and immunoblotting revealed that XIAP, c-IAP1 and c-IAP2 are down-regulated in pregnancy and lactation, and prior to the onset of involution. In cultured mammary epithelial cells (MECs), XIAP levels decreased in response to inhibition of growth factor signalling. Maintaining XIAP levels in MECs by expressing exogenous XIAP protected them from all apoptotic stimuli tested. Conclusions. These data suggest that the developmental regulation of IAP expression in vivo contributes to naturally occurring programmes of cell death.
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U2 - 10.1186/1471-213X-10-71
DO - 10.1186/1471-213X-10-71
M3 - Article
C2 - 20584313
AN - SCOPUS:77953946920
SN - 1471-213X
VL - 10
JO - BMC Developmental Biology
JF - BMC Developmental Biology
M1 - 71
ER -