Analysis of inter-(simple sequence repeat) PCR products from human colorectal cancers

Neng Chen; Daniel L. Stoler; Smitha S. Dutt; Gerald P. Jahreis; Miguel A. Rodriguez-Bigas; Nicholas J. Petrelli; Garth R. Anderson

doi:10.3727/096504005776382297

Analysis of inter-(simple sequence repeat) PCR products from human colorectal cancers

Neng Chen, Daniel L. Stoler, Smitha S. Dutt, Gerald P. Jahreis, Miguel A. Rodriguez-Bigas, Nicholas J. Petrelli, Garth R. Anderson

Surgical Oncology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.

Original language	English (US)
Pages (from-to)	233-238
Number of pages	6
Journal	Oncology research
Volume	15
Issue number	4
DOIs	https://doi.org/10.3727/096504005776382297
State	Published - 2005

Keywords

Genomic instability
Instability events
Inter-(simple sequence repeat) PCR

ASJC Scopus subject areas

Oncology
Cancer Research

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10.3727/096504005776382297

Cite this

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abstract = "Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.",

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AU - Stoler, Daniel L.

AU - Dutt, Smitha S.

AU - Jahreis, Gerald P.

AU - Rodriguez-Bigas, Miguel A.

AU - Petrelli, Nicholas J.

AU - Anderson, Garth R.

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AB - Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.

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Analysis of inter-(simple sequence repeat) PCR products from human colorectal cancers

Abstract

Keywords

ASJC Scopus subject areas

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