TY - JOUR
T1 - Aneuploidy index in blood
T2 - A potential marker for early onset, androgen response, and metastasis in human prostate cancer
AU - Ozen, Mustafa
AU - Hopwood, Vicki L.
AU - Johnston, Dennis A.
AU - Babaian, Richard J.
AU - Logothetis, Christopher J.
AU - Von Eschenbach, Andrew C.
AU - Pathak, Sen
N1 - Funding Information:
This work was supported in part by grants from the John S. Dunn Research Foundation of Houston, Institutional Prostate Cancer Research Program, and NIH grant RRO 4999-01. Mustafa Ozen was supported by a scholarship from the Pamukkale University, Denizli, Turkey.
PY - 1999/2
Y1 - 1999/2
N2 - Objectives. To investigate whether the frequency of chromosome abnormalities in peripheral blood lymphocytes defined as the aneuploidy index in blood (AnlB) can be used as a clinical marker of early age onset, androgen response, and metastasis in human prostate cancer. Methods. Peripheral blood samples were collected from 80 patients with prostate cancer, and chromosome preparations were made from 72-hour cultures after mitotic block. The AnlB of 59 informative cases was compared with several parameters, including age at disease onset, Gleason grade of tumor, clinical stage of tumor, metastasis, and prostate-specific antigen (PSA) level. Results. Patients with AnlB levels greater than 3 had a significantly higher incidence of metastasis (P = 0.022), androgen-independent disease (P = 0.002), and early age at disease onset (age at diagnosis less than 65 years) (P = 0.002) compared with the patients with lower AnlB (less than 3) levels. In addition, patients with AnlB levels greater than 5 had higher PSA levels (greater than 20 ng/mL) (P = 0.029) than patients with AnlB levels less than 5. Conclusions. Chromosome abnormalities can be detected in the peripheral lymphocytes of patients with prostate cancer, and AnlB can be used as an early diagnostic and predictive marker for prostate cancer metastasis and androgen-independent disease.
AB - Objectives. To investigate whether the frequency of chromosome abnormalities in peripheral blood lymphocytes defined as the aneuploidy index in blood (AnlB) can be used as a clinical marker of early age onset, androgen response, and metastasis in human prostate cancer. Methods. Peripheral blood samples were collected from 80 patients with prostate cancer, and chromosome preparations were made from 72-hour cultures after mitotic block. The AnlB of 59 informative cases was compared with several parameters, including age at disease onset, Gleason grade of tumor, clinical stage of tumor, metastasis, and prostate-specific antigen (PSA) level. Results. Patients with AnlB levels greater than 3 had a significantly higher incidence of metastasis (P = 0.022), androgen-independent disease (P = 0.002), and early age at disease onset (age at diagnosis less than 65 years) (P = 0.002) compared with the patients with lower AnlB (less than 3) levels. In addition, patients with AnlB levels greater than 5 had higher PSA levels (greater than 20 ng/mL) (P = 0.029) than patients with AnlB levels less than 5. Conclusions. Chromosome abnormalities can be detected in the peripheral lymphocytes of patients with prostate cancer, and AnlB can be used as an early diagnostic and predictive marker for prostate cancer metastasis and androgen-independent disease.
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U2 - 10.1016/S0090-4295(98)00487-7
DO - 10.1016/S0090-4295(98)00487-7
M3 - Article
C2 - 9933059
AN - SCOPUS:0032895242
SN - 0090-4295
VL - 53
SP - 381
EP - 385
JO - Urology
JF - Urology
IS - 2
ER -