TY - JOUR
T1 - Annexin A2 associates to feline calicivirus RNA in the replication complexes from infected cells and participates in an efficient viral replication
AU - Santos-Valencia, Juan Carlos
AU - Cancio-Lonches, Clotilde
AU - Trujillo-Uscanga, Adrian
AU - Alvarado-Hernández, Beatriz
AU - Lagunes-Guillén, Anel
AU - Gutiérrez-Escolano, Ana Lorena
N1 - Funding Information:
This work was supported by Consejo Nacional de Ciencia y Tecnología, grant number 0250696.
Funding Information:
This work was supported by Consejo Nacional de Ciencia y Tecnología , grant number 0250696 .
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/2
Y1 - 2019/2
N2 - Cellular proteins have been identified to participate in calicivirus replication in association with viral proteins and/or viral RNAs. By mass spectrometry from pull-down assays, we identified several cellular proteins bound to the feline calicivirus (FCV) genomic RNA; among them the lipid raft-associated scaffold protein Annexin (Anx) A2. AnxA2 colocalizes with FCV NS6/7 protein and with the dsRNA in infected cells; moreover, it was found associated with the viral RNA in the membrane fraction corresponding to the replication complexes (RCs), suggesting its role during FCV replication. AnxA2-knockdown from CrFK cells prior to infection with FCV caused a delay in the cytopathic effect, a strong reduction of viral non-structural proteins and dsRNA production, and a decrease of FCV yield in both cell-associated and supernatant fractions. Taken together, these results indicate that AnxA2 associates to the genomic RNA of FCV and is required for an efficient FCV replication.
AB - Cellular proteins have been identified to participate in calicivirus replication in association with viral proteins and/or viral RNAs. By mass spectrometry from pull-down assays, we identified several cellular proteins bound to the feline calicivirus (FCV) genomic RNA; among them the lipid raft-associated scaffold protein Annexin (Anx) A2. AnxA2 colocalizes with FCV NS6/7 protein and with the dsRNA in infected cells; moreover, it was found associated with the viral RNA in the membrane fraction corresponding to the replication complexes (RCs), suggesting its role during FCV replication. AnxA2-knockdown from CrFK cells prior to infection with FCV caused a delay in the cytopathic effect, a strong reduction of viral non-structural proteins and dsRNA production, and a decrease of FCV yield in both cell-associated and supernatant fractions. Taken together, these results indicate that AnxA2 associates to the genomic RNA of FCV and is required for an efficient FCV replication.
KW - Annexin A2
KW - Feline calicivirus
KW - Replication complexes
KW - RNA-protein interaction
KW - Viral replication
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U2 - 10.1016/j.virusres.2018.12.003
DO - 10.1016/j.virusres.2018.12.003
M3 - Article
C2 - 30543874
AN - SCOPUS:85058474583
SN - 0168-1702
VL - 261
SP - 1
EP - 8
JO - Virus Research
JF - Virus Research
ER -