TY - JOUR
T1 - Annona muricata Leaves as a Source of Bioactive Compounds
T2 - Extraction and Quantification Using Ultrasound
AU - Nolasco-González, Yolanda
AU - Chacón-López, Martina Alejandra
AU - Ortiz-Basurto, Rosa Isela
AU - Aguilera-Aguirre, Selene
AU - González-Aguilar, Gustavo Adolfo
AU - Rodríguez-Aguayo, Cristian
AU - Navarro-Cortez, Merle Catalina
AU - García-Galindo, Hugo Sergio
AU - García-Magaña, María de Lourdes
AU - Meza-Espinoza, Libier
AU - Montalvo-González, Efigenia
N1 - Funding Information:
The authors thank CONACYT-MEXICO for scholarship 780496 for Yolanda Nolasco González. This research is part of the activities of the National Networks “Anonáceas” and “Reduce and Valorize Food Losses and Waste Towards Sustainable Food Systems (294768)”.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/7
Y1 - 2022/7
N2 - In this work, the ultrasound-assisted extraction (UAE) (operating conditions of sonication amplitude, pulse-cycle, and extraction time) was investigated to obtain an extract rich in biologically active compounds from Annona muricata leaves. In addition, the content of bioactive compounds from the extract by the optimal UAE conditions (UAE extract) was compared with extracts prepared by decoction and infusion. Moreover, Artemia salina toxicity was evaluated in all extracts. UAE extract (using optimal conditions: 80% amplitude, 0.7 s pulse-cycle, for 4.54 min) contained 178.48 mg/100 mL of soluble phenols, 20.18 mg/100 mL of total flavonoids, 27.81 mg/100 mL of hy-drolyzable polyphenols, 167.07 mg/100 mL of condensed tannins, 30.44 mg/100 mL of total alkaloids and 14.62 mg/100 mL of total acetogenins. The UAE extract exhibited a high antioxidant capacity and a higher content of bioactive compounds from ~6 to ~927-fold than decoction or infusion, depending on the type of compound. Twenty-four different phenolic compounds were identified in the samples, and UAE extract produced the highest concentration. All extracts were considered non-toxic using the A. salina test. The UAE extract from A. muricata leaves is a rich source of bioactive compounds and can be utilized to formulate therapeutic drugs or nutraceutical foods.
AB - In this work, the ultrasound-assisted extraction (UAE) (operating conditions of sonication amplitude, pulse-cycle, and extraction time) was investigated to obtain an extract rich in biologically active compounds from Annona muricata leaves. In addition, the content of bioactive compounds from the extract by the optimal UAE conditions (UAE extract) was compared with extracts prepared by decoction and infusion. Moreover, Artemia salina toxicity was evaluated in all extracts. UAE extract (using optimal conditions: 80% amplitude, 0.7 s pulse-cycle, for 4.54 min) contained 178.48 mg/100 mL of soluble phenols, 20.18 mg/100 mL of total flavonoids, 27.81 mg/100 mL of hy-drolyzable polyphenols, 167.07 mg/100 mL of condensed tannins, 30.44 mg/100 mL of total alkaloids and 14.62 mg/100 mL of total acetogenins. The UAE extract exhibited a high antioxidant capacity and a higher content of bioactive compounds from ~6 to ~927-fold than decoction or infusion, depending on the type of compound. Twenty-four different phenolic compounds were identified in the samples, and UAE extract produced the highest concentration. All extracts were considered non-toxic using the A. salina test. The UAE extract from A. muricata leaves is a rich source of bioactive compounds and can be utilized to formulate therapeutic drugs or nutraceutical foods.
KW - Annona muricata leaves
KW - bioactive compounds
KW - ultrasound-assisted extraction
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U2 - 10.3390/horticulturae8070560
DO - 10.3390/horticulturae8070560
M3 - Article
AN - SCOPUS:85132921400
SN - 2311-7524
VL - 8
JO - Horticulturae
JF - Horticulturae
IS - 7
M1 - 560
ER -