TY - JOUR
T1 - Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways
AU - Kim, Kyungjae
AU - Lee, Sungwon
AU - Shin, Seulmee
AU - Kim, Hyunyul
AU - Han, Shinha
AU - Kim, Kwanghee
AU - Kwon, Jeunghak
AU - Kwak, Jin Hwan
AU - Lee, Chong Kil
AU - Ha, Nam Joo
AU - Yim, Dongsool
N1 - Funding Information:
This study was supported by the Sahmyook University Research fund in 2010.
PY - 2011
Y1 - 2011
N2 - Background: Arctiin, isolated from Forsythia suspensa has been reported to have anti-inflammatory, anti-oxidant, antibacterial, and antiviral effects in vitro. However, there has been a lack of studies regarding its effects on immunological activity. The aim of this study is to investigate the anti-inflammatory potential and possible mechanisms of arctiin in LPS-induced macrophages. Methods. We investigated the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecule changes. Results: Arctiin dose dependently decreased the production of NO and proinflammatory cytokines such as IL-1, IL-6, TNF-, and PGE2, and it reduced the gene and protein levels as determined by RT-PCR and western blot analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 were also inhibited by arctiin. Furthermore, the activation of the nuclear transcription factor, NF-B in macrophages was inhibited by arctiin. Conclusion: Taken together these results provide evidence of the bioactivity of arctiin in inflammatory diseases and suggest that arctiin may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators through the inactivation of NF-kB.
AB - Background: Arctiin, isolated from Forsythia suspensa has been reported to have anti-inflammatory, anti-oxidant, antibacterial, and antiviral effects in vitro. However, there has been a lack of studies regarding its effects on immunological activity. The aim of this study is to investigate the anti-inflammatory potential and possible mechanisms of arctiin in LPS-induced macrophages. Methods. We investigated the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecule changes. Results: Arctiin dose dependently decreased the production of NO and proinflammatory cytokines such as IL-1, IL-6, TNF-, and PGE2, and it reduced the gene and protein levels as determined by RT-PCR and western blot analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 were also inhibited by arctiin. Furthermore, the activation of the nuclear transcription factor, NF-B in macrophages was inhibited by arctiin. Conclusion: Taken together these results provide evidence of the bioactivity of arctiin in inflammatory diseases and suggest that arctiin may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators through the inactivation of NF-kB.
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U2 - 10.1186/1476-9255-8-16
DO - 10.1186/1476-9255-8-16
M3 - Article
C2 - 21733191
AN - SCOPUS:79959969209
SN - 1476-9255
VL - 8
JO - Journal of Inflammation
JF - Journal of Inflammation
M1 - 16
ER -