TY - JOUR
T1 - Antiapoptotic role for ornithine decarboxylase during oocyte maturation
AU - Zhou, Yong
AU - Ma, Chunqi
AU - Karmouch, Jennifer
AU - Katbi, Hadia Arabi
AU - Liu, X. Johné
PY - 2009/4
Y1 - 2009/4
N2 - Ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis, is a nonredundant and essential gene in all eukaryotes. During the mitotic cell cycle, ODC exhibits two activity peaks: one at the G1/S transition and one during the G2/M transition. The physiological role of this cell cycle-dependent ODC activity dynamic is not clear. Previous studies have reported a significant elevation of ODC activity during Xenopus oocyte maturation, which resembles mitotic G2/M transition. In order to study the roles of ODC activity in the oocytes, we utilized antisense morpholino (xODC mo) oligonucleotides to inhibit ODC translation. We report here that xODC mo abolished ODC activity increase during oocyte maturation. xODC mo-injected oocytes underwent germinal vesicle breakdown, emitted the first polar body, and reached metaphase II, thus completing nuclear maturation. However, the metaphase II oocytes exhibited high levels of reactive oxygen species and became apoptotic. When transferred to host frogs and subsequently ovulated, these eggs were fertilized but exhibited embryo fragmentation. Translation of ODC is therefore integral to cytoplasmic maturation, protecting metaphase II oocytes from reactive oxygen species-induced apoptosis.
AB - Ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis, is a nonredundant and essential gene in all eukaryotes. During the mitotic cell cycle, ODC exhibits two activity peaks: one at the G1/S transition and one during the G2/M transition. The physiological role of this cell cycle-dependent ODC activity dynamic is not clear. Previous studies have reported a significant elevation of ODC activity during Xenopus oocyte maturation, which resembles mitotic G2/M transition. In order to study the roles of ODC activity in the oocytes, we utilized antisense morpholino (xODC mo) oligonucleotides to inhibit ODC translation. We report here that xODC mo abolished ODC activity increase during oocyte maturation. xODC mo-injected oocytes underwent germinal vesicle breakdown, emitted the first polar body, and reached metaphase II, thus completing nuclear maturation. However, the metaphase II oocytes exhibited high levels of reactive oxygen species and became apoptotic. When transferred to host frogs and subsequently ovulated, these eggs were fertilized but exhibited embryo fragmentation. Translation of ODC is therefore integral to cytoplasmic maturation, protecting metaphase II oocytes from reactive oxygen species-induced apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=63049090705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63049090705&partnerID=8YFLogxK
U2 - 10.1128/MCB.01815-08
DO - 10.1128/MCB.01815-08
M3 - Article
C2 - 19158268
AN - SCOPUS:63049090705
SN - 0270-7306
VL - 29
SP - 1786
EP - 1795
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 7
ER -