TY - JOUR
T1 - Antibodies against the T cell receptor/CD3 complex interfere with distinct intra‐thymic cell‐cell interactions in vivo
T2 - correlation with arrest of T cell differentiation
AU - Kyewski, Bruno A.
AU - Schirrmacher, Volker
AU - Allison, James P.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989/5
Y1 - 1989/5
N2 - Postnatal treatment of mice with antibodies against the T cell receptor complex (TcR) prevents the differentiation of mature T cells in the thymic medulla without affecting the generation of most immature cortical thymocytes, thus interfering with a discrete stage of intra‐thymic T cell differentiation at the cortex/medulla transition. This result has been interpreted as indicating a direct role of the TcR in the differentiation of immature to mature T cells, possibly via TcR‐ligand interactions during direct cell‐cell contact. Here we analyze the effect of anti‐TcR (Vβ8 family) and anti‐CD3 (ε chain) antibodies on distinct intra‐thymic cell‐cell interactions in vivo. We find that the maturation arrest of thymocytes correlates with a nearly complete abrogation of interactions of corresponding immature thymocyte with I‐A/E+ cortical epithelial cells and I‐A/E+ medullary dendritic cells, while preserving interactions with adherent I‐A/E− macrophages. It is proposed that the blockade of thymocyte‐epithelial cell recognition in the cortex by anti‐TcR antibodies prevents the translocation of thymocytes into the medulla and their subsequent differentiation and selection, including interactions with dendritic cells. Interestingly, the anti‐CD3 mAb treatment seems to spare the intra‐thymic development of the CD3+, CD4−/CD8− T cell lineage.
AB - Postnatal treatment of mice with antibodies against the T cell receptor complex (TcR) prevents the differentiation of mature T cells in the thymic medulla without affecting the generation of most immature cortical thymocytes, thus interfering with a discrete stage of intra‐thymic T cell differentiation at the cortex/medulla transition. This result has been interpreted as indicating a direct role of the TcR in the differentiation of immature to mature T cells, possibly via TcR‐ligand interactions during direct cell‐cell contact. Here we analyze the effect of anti‐TcR (Vβ8 family) and anti‐CD3 (ε chain) antibodies on distinct intra‐thymic cell‐cell interactions in vivo. We find that the maturation arrest of thymocytes correlates with a nearly complete abrogation of interactions of corresponding immature thymocyte with I‐A/E+ cortical epithelial cells and I‐A/E+ medullary dendritic cells, while preserving interactions with adherent I‐A/E− macrophages. It is proposed that the blockade of thymocyte‐epithelial cell recognition in the cortex by anti‐TcR antibodies prevents the translocation of thymocytes into the medulla and their subsequent differentiation and selection, including interactions with dendritic cells. Interestingly, the anti‐CD3 mAb treatment seems to spare the intra‐thymic development of the CD3+, CD4−/CD8− T cell lineage.
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U2 - 10.1002/eji.1830190512
DO - 10.1002/eji.1830190512
M3 - Article
C2 - 2525475
AN - SCOPUS:0024327233
SN - 0014-2980
VL - 19
SP - 857
EP - 863
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 5
ER -