TY - JOUR
T1 - Antral atrophy, intestinal metaplasia, and preneoplastic markers in Mexican children with Helicobacter pylori-positive and Helicobacter pylori-negative gastritis
AU - Villarreal-Calderon, Rodolfo
AU - Luévano-González, Arturo
AU - Aragón-Flores, Mariana
AU - Zhu, Hongtu
AU - Yuan, Ying
AU - Xiang, Qun
AU - Yan, Benjamin
AU - Stoll, Kathryn Anne
AU - Cross, Janet V.
AU - Iczkowski, Kenneth A.
AU - Mackinnon, Alexander Craig
N1 - Funding Information:
The Federation of American Societies for Experimental Biology, the American Society for Investigative Pathology, and their Minority Access to Research Careers Summer Research Opportunities Program in Pathology generously supported Rodolfo Villarreal-Calderon to conduct research for this study at the Medical College of Wisconsin in Milwaukee, WI, under the mentorship of Dr Alexander Craig Mackinnon, Jr. This project was supported, in part, by grant 1UL1RR031973 from the Clinical and Translational Science Award program of the National Center for Research Resources, National Institutes of Health , which allowed for the biostatistics work at the Medical College of Wisconsin Institute for Health and Society, with the help of Assistant Professor Dr Sergey Tarima.
PY - 2014/6
Y1 - 2014/6
N2 - Chronic inflammation and infection are major risk factors for gastric carcinogenesis in adults. As chronic gastritis is common in Mexican children, diagnosis of Helicobacter pylori and other causes of gastritis are critical for the identification of children who would benefit from closer surveillance. Antral biopsies from 82 Mexican children (mean age, 8.3 ± 4.8 years) with chronic gastritis (36 H pylori+, 46 H pylori-) were examined for gastritis activity, atrophy, intestinal metaplasia (IM), and immunohistochemical expression of gastric carcinogenesis biomarkers caudal type homeobox 2 (CDX2), ephrin type-B receptor 4 (EphB4), matrix metalloproteinase 3 (MMP3), macrophage migration inhibitory factor (MIF), p53, β-catenin, and E-cadherin. Atrophy was diagnosed in 7 (9%) of 82, and IM, in 5 (6%) of 82 by routine histology, whereas 6 additional children (7%) (3 H pylori+) exhibited aberrant CDX2 expression without IM. Significant positive correlations were seen between EphB4, MMP3, and MIF (P <.0001). Atrophy and follicular pathology were more frequent in H pylori+ biopsies (P <.0001), whereas IM and CDX2 expression showed no significant correlation with H pylori status. Antral biopsies demonstrating atrophy, IM, and/or aberrant CDX2 expression were seen in 21.95% (18/82) of the children, potentially identifying those who would benefit from closer surveillance and preventive dietary strategies. Biomarkers CDX2, EphB4, MMP3, and MIF may be useful in the workup of pediatric gastritis.
AB - Chronic inflammation and infection are major risk factors for gastric carcinogenesis in adults. As chronic gastritis is common in Mexican children, diagnosis of Helicobacter pylori and other causes of gastritis are critical for the identification of children who would benefit from closer surveillance. Antral biopsies from 82 Mexican children (mean age, 8.3 ± 4.8 years) with chronic gastritis (36 H pylori+, 46 H pylori-) were examined for gastritis activity, atrophy, intestinal metaplasia (IM), and immunohistochemical expression of gastric carcinogenesis biomarkers caudal type homeobox 2 (CDX2), ephrin type-B receptor 4 (EphB4), matrix metalloproteinase 3 (MMP3), macrophage migration inhibitory factor (MIF), p53, β-catenin, and E-cadherin. Atrophy was diagnosed in 7 (9%) of 82, and IM, in 5 (6%) of 82 by routine histology, whereas 6 additional children (7%) (3 H pylori+) exhibited aberrant CDX2 expression without IM. Significant positive correlations were seen between EphB4, MMP3, and MIF (P <.0001). Atrophy and follicular pathology were more frequent in H pylori+ biopsies (P <.0001), whereas IM and CDX2 expression showed no significant correlation with H pylori status. Antral biopsies demonstrating atrophy, IM, and/or aberrant CDX2 expression were seen in 21.95% (18/82) of the children, potentially identifying those who would benefit from closer surveillance and preventive dietary strategies. Biomarkers CDX2, EphB4, MMP3, and MIF may be useful in the workup of pediatric gastritis.
KW - Antral gastritis
KW - Biomarkers
KW - Child
KW - Helicobacter pylori
KW - Mexican
KW - Surveillance
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U2 - 10.1016/j.anndiagpath.2014.02.003
DO - 10.1016/j.anndiagpath.2014.02.003
M3 - Article
C2 - 24656654
AN - SCOPUS:84901198233
SN - 1092-9134
VL - 18
SP - 129
EP - 135
JO - Annals of Diagnostic Pathology
JF - Annals of Diagnostic Pathology
IS - 3
ER -