Appearance of Glutamate Receptors on the Surface of HL-60 Cells upon Differentiation

I. A. Kostanyan; R. I. Nurieva; T. N. Lepikhova; M. V. Astapova; E. V. Navolotskaya; V. P. Zav'yalov; V. M. Lipkin

Appearance of Glutamate Receptors on the Surface of HL-60 Cells upon Differentiation

I. A. Kostanyan, R. I. Nurieva, T. N. Lepikhova, M. V. Astapova, E. V. Navolotskaya, V. P. Zav'yalov, V. M. Lipkin

Research output: Contribution to journal › Article › peer-review

Abstract

A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (K_d = 0.5 μM) and by plasma membrane fraction from the same cells (K_d = 1 μM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue ot glutamic acid, was found to inhibit competitively the specific binding of L-[³H]glutamic acid to the membranes (K_i = 0.24 μM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.

Original language	English (US)
Pages (from-to)	470
Number of pages	1
Journal	Bioorganicheskaya Khimiya
Volume	24
Issue number	6
State	Published - 1998
Externally published	Yes

Keywords

All-E-retinoic acid
Differentiation
Glutamate receptors
HL-60 cells
L-glutamic acid

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Appearance of Glutamate Receptors on the Surface of HL-60 Cells upon Differentiation",

abstract = "A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 μM) and by plasma membrane fraction from the same cells (Kd = 1 μM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue ot glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 μM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.",

keywords = "All-E-retinoic acid, Differentiation, Glutamate receptors, HL-60 cells, L-glutamic acid",

author = "Kostanyan, {I. A.} and Nurieva, {R. I.} and Lepikhova, {T. N.} and Astapova, {M. V.} and Navolotskaya, {E. V.} and Zav'yalov, {V. P.} and Lipkin, {V. M.}",

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language = "English (US)",

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TY - JOUR

T1 - Appearance of Glutamate Receptors on the Surface of HL-60 Cells upon Differentiation

AU - Kostanyan, I. A.

AU - Nurieva, R. I.

AU - Lepikhova, T. N.

AU - Astapova, M. V.

AU - Navolotskaya, E. V.

AU - Zav'yalov, V. P.

AU - Lipkin, V. M.

PY - 1998

Y1 - 1998

N2 - A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 μM) and by plasma membrane fraction from the same cells (Kd = 1 μM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue ot glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 μM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.

AB - A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 μM) and by plasma membrane fraction from the same cells (Kd = 1 μM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue ot glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 μM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.

KW - All-E-retinoic acid

KW - Differentiation

KW - Glutamate receptors

KW - HL-60 cells

KW - L-glutamic acid

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Appearance of Glutamate Receptors on the Surface of HL-60 Cells upon Differentiation

Abstract

Keywords

ASJC Scopus subject areas

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