Abstract
A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 mkM) and by plasma membrane fraction from the same cells (Kd = 1 mkM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue of glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 μM). The stereospecificity of the binding was demonstrated. The data obtained suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.
Original language | English (US) |
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Pages (from-to) | 411-412 |
Number of pages | 2 |
Journal | Russian Journal of Bioorganic Chemistry |
Volume | 24 |
Issue number | 6 |
State | Published - 1998 |
Externally published | Yes |
Keywords
- Differentiation
- HL-60 cells
- L-glutamic acid
- all-E-retinoic acid
- glutamate receptors
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry