Poiavlenie glutamatnykh retseptorov na poverkhnosti kletok HL-60 pri ikh differentsirovke.

I. A. Kostanian; Roza Nurieva; T. N. Lepikhova; M. V. Astapova; E. V. Navolotskaia; V. P. Zavialov; V. M. Lipkin

Poiavlenie glutamatnykh retseptorov na poverkhnosti kletok HL-60 pri ikh differentsirovke.

Translated title of the contribution: Appearance of glutamate receptors on the surface of HL-60 cells upon differentiation

I. A. Kostanian, Roza Nurieva, T. N. Lepikhova, M. V. Astapova, E. V. Navolotskaia, V. P. Zavialov, V. M. Lipkin

Immunology

Research output: Contribution to journal › Letter › peer-review

Abstract

A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 microM) and by plasma membrane fraction from the same cells (Kd = 1 microM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue of glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 microM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.

Translated title of the contribution	Appearance of glutamate receptors on the surface of HL-60 cells upon differentiation
Original language	Russian
Pages (from-to)	468-470
Number of pages	3
Journal	Bioorganicheskaia khimiia
Volume	24
Issue number	6
State	Published - Jun 1998

ASJC Scopus subject areas

Organic Chemistry
General Biochemistry, Genetics and Molecular Biology

Cite this

@article{2c13071ddfcd41569b304960221efe9e,

title = "Poiavlenie glutamatnykh retseptorov na poverkhnosti kletok HL-60 pri ikh differentsirovke.",

abstract = "A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 microM) and by plasma membrane fraction from the same cells (Kd = 1 microM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue of glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 microM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.",

author = "Kostanian, {I. A.} and Roza Nurieva and Lepikhova, {T. N.} and Astapova, {M. V.} and Navolotskaia, {E. V.} and Zavialov, {V. P.} and Lipkin, {V. M.}",

year = "1998",

month = jun,

language = "Russian",

volume = "24",

pages = "468--470",

journal = "Bioorganicheskaia khimiia",

issn = "0132-3423",

publisher = "Izdatel'stvo Nauka",

number = "6",

}

TY - JOUR

T1 - Poiavlenie glutamatnykh retseptorov na poverkhnosti kletok HL-60 pri ikh differentsirovke.

AU - Kostanian, I. A.

AU - Nurieva, Roza

AU - Lepikhova, T. N.

AU - Astapova, M. V.

AU - Navolotskaia, E. V.

AU - Zavialov, V. P.

AU - Lipkin, V. M.

PY - 1998/6

Y1 - 1998/6

N2 - A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 microM) and by plasma membrane fraction from the same cells (Kd = 1 microM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue of glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 microM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.

AB - A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 microM) and by plasma membrane fraction from the same cells (Kd = 1 microM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue of glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 microM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0032088178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032088178&partnerID=8YFLogxK

M3 - Letter

C2 - 9702357

SN - 0132-3423

VL - 24

SP - 468

EP - 470

JO - Bioorganicheskaia khimiia

JF - Bioorganicheskaia khimiia

IS - 6

ER -

Poiavlenie glutamatnykh retseptorov na poverkhnosti kletok HL-60 pri ikh differentsirovke.

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this