Aptasensors for rapid detection of Escherichia coli O157: H7 and Salmonella typhimurium

Wen He Wu, Min Li, Yue Wang, Hou Xian Ouyang, Lin Wang, Xiu Ci Li, Yu Chen Cao, Qing He Meng, Jian Xin Lu

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

Herein we reported the development of aptamer-based biosensors (aptasensors) based on label-free aptamers and gold nanoparticles (AuNPs) for detection of Escherichia coli (E. coli) O157:H7 and Salmonella typhimurium. Target bacteria binding aptamers are adsorbed on the surface of unmodified AuNPs to capture target bacteria, and the detection was accomplished by target bacteria-induced aggregation of the aptasensor which is associated as red-to-purple color change upon high-salt conditions. By employing anti-E. coli O157:H7 aptamer and anti-S. typhimurium aptamer, we developed a convenient and rapid approach that could selectively detect bacteria without specialized instrumentation and pretreatment steps such as cell lysis. The aptasensor could detect as low as 105 colony-forming units (CFU)/ml target bacteria within 20 min or less and its specificity was 100%. This novel method has a great potential application in rapid detection of bacteria in the near future.

Original languageEnglish (US)
Article number658
JournalNanoscale Research Letters
Volume7
DOIs
StatePublished - 2012

Keywords

  • Aptasensor
  • AuNPs
  • Colorimetric detection
  • E. coli O157:H7
  • Salmonella typhimurium

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics

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