Asbestos-associated chromosomal changes in human mesothelial cells

J. F. Lechner; T. Tokiwa; M. LaVeck; W. F. Benedict; S. Banks-Schlegel; H. Yeager; A. Banerjee; C. C. Harris

doi:10.1073/pnas.82.11.3884

Asbestos-associated chromosomal changes in human mesothelial cells

J. F. Lechner, T. Tokiwa, M. LaVeck, W. F. Benedict, S. Banks-Schlegel, H. Yeager, A. Banerjee, C. C. Harris

Genitourinary Medical Oncology

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Abstract

Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than norman human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.

Original language	English (US)
Pages (from-to)	3884-3888
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	82
Issue number	11
DOIs	https://doi.org/10.1073/pnas.82.11.3884
State	Published - 1985

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title = "Asbestos-associated chromosomal changes in human mesothelial cells",

abstract = "Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than norman human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.",

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T1 - Asbestos-associated chromosomal changes in human mesothelial cells

AU - Lechner, J. F.

AU - Tokiwa, T.

AU - LaVeck, M.

AU - Benedict, W. F.

AU - Banks-Schlegel, S.

AU - Yeager, H.

AU - Banerjee, A.

AU - Harris, C. C.

PY - 1985

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N2 - Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than norman human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.

AB - Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than norman human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.

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Asbestos-associated chromosomal changes in human mesothelial cells

Abstract

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