Abstract
Aurora-A kinase is considered a potential cancer susceptibility gene that encodes a centrosome-associated, cell cycle-regulated serine/threonine kinase. We studied two single nucleotide polymorphisms (SNP) in the coding region of Aurora-A, 91T-to-A (F31I) and 169G-to-A (V57I). We studied the influence of these two polymorphisms on age of onset of hereditary nonpolyposis colorectal cancer (HNPCC). Genotyping of the Aurora-A polymorphisms was carried out on 125 Caucasian with mismatch repair (MMR) gene mutations with real-time pyrophosphate DNA sequencing. For the 91T-to-A polymorphism, we found that patients with HNPCC who were homozygous for the wild-type allele developed colorectal cancer (CRC) 7 years earlier than patients who were homozygous or heterozygous for the mutant allele. The 169G-to-A polymorphism did not have a significant influence on risk for HNPCC. However, when we did haplotype analysis for these two polymorphisms, the 91A-169G haplotype was associated with protection from HNPCC at an earlier age.
Original language | English (US) |
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Pages (from-to) | 249-256 |
Number of pages | 8 |
Journal | Molecular Carcinogenesis |
Volume | 46 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
Keywords
- Age of onset
- Aurora-A kinase
- HNPCC
- Haplotype
- Polymorphism
ASJC Scopus subject areas
- Molecular Biology
- Cancer Research