TY - JOUR
T1 - Autocrine interleukin-1β production in leukemia
T2 - Evidence for the involvement of mutated RAS
AU - Beaupre, Darrin M.
AU - Talpaz, Moshe
AU - Marini, Frank C.
AU - Cristiano, Richard J.
AU - Roth, Jack A.
AU - Estrov, Zeev
AU - Albitar, Maher
AU - Freedman, Melvin H.
AU - Kurzrock, Razelle
N1 - Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 1999/6/15
Y1 - 1999/6/15
N2 - Interleukin (IL)-1β is constitutively expressed in many leukemias and operates as an autocrine growth factor. To study the cellular basis for this aberrant production, we analyzed two cell lines, B1 (acute lymphoblastic leukemia) and W1 (juvenile chronic myelogenous leukemia), which express high levels of IL-1β and have mutations in the K-RAS and N-RAS genes, respectively. Electromobility shift assays demonstrated transcription factor binding at multiple IL-1β promoter elements [nuclear factor (NF)-IL6/CREB, NFB1, NFκB, and NF-IL6], consistent with the activation of an upstream signaling pathway. To determine whether activated Ras was involved, two structurally distinct classes of farnesyltransferase (FTase) inhibitors (the monoterpenes and a peptidomimetic) and an adenoviral vector expressing antisense targeted to K-RAS were used to specifically interfere with Ras function and/or expression. Treatment with the FTase inhibitors resulted in a concentration-dependent decrease in both NF-IL6/CREB binding to the IL-1β promoter and IL-1β protein levels, without a significant change in total cellular protein levels. Furthermore, exposure of the B1 cells to antisense against K-RAS resulted in an approximately 50% reduction in both p21(Ras) and IL-1β protein levels. Growth suppression was observed after FTase inhibitor or antisense exposure, an effect that was partially reversible by the addition of recombinant IL-1β to the cultures. Our observations suggest that mutated RAS genes may mediate autocrine IL-1β production in some leukemias by stimulating signal transduction pathways thai activate the IL-1β promoter.
AB - Interleukin (IL)-1β is constitutively expressed in many leukemias and operates as an autocrine growth factor. To study the cellular basis for this aberrant production, we analyzed two cell lines, B1 (acute lymphoblastic leukemia) and W1 (juvenile chronic myelogenous leukemia), which express high levels of IL-1β and have mutations in the K-RAS and N-RAS genes, respectively. Electromobility shift assays demonstrated transcription factor binding at multiple IL-1β promoter elements [nuclear factor (NF)-IL6/CREB, NFB1, NFκB, and NF-IL6], consistent with the activation of an upstream signaling pathway. To determine whether activated Ras was involved, two structurally distinct classes of farnesyltransferase (FTase) inhibitors (the monoterpenes and a peptidomimetic) and an adenoviral vector expressing antisense targeted to K-RAS were used to specifically interfere with Ras function and/or expression. Treatment with the FTase inhibitors resulted in a concentration-dependent decrease in both NF-IL6/CREB binding to the IL-1β promoter and IL-1β protein levels, without a significant change in total cellular protein levels. Furthermore, exposure of the B1 cells to antisense against K-RAS resulted in an approximately 50% reduction in both p21(Ras) and IL-1β protein levels. Growth suppression was observed after FTase inhibitor or antisense exposure, an effect that was partially reversible by the addition of recombinant IL-1β to the cultures. Our observations suggest that mutated RAS genes may mediate autocrine IL-1β production in some leukemias by stimulating signal transduction pathways thai activate the IL-1β promoter.
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M3 - Article
C2 - 10383163
AN - SCOPUS:0033563322
SN - 0008-5472
VL - 59
SP - 2971
EP - 2980
JO - Cancer Research
JF - Cancer Research
IS - 12
ER -