@inproceedings{432e3ef5e93445bda15892533cb7a629,
title = "Automated analysis of human protein atlas immunofluorescence images",
abstract = "The Human Protein Atlas is a rich source of location proteomics data. In this work, we present an automated approach for processing and classifying major subcellular patterns in the Atlas images. We demonstrate that two different classification frameworks (support vector machine and random forest) are effective at determining subcellular locations; we can analyze over 3500 Atlas images with a high degree of accuracy, up to 87.5% for all of the samples and 98.5% when only considering samples in whose classification assignments we are most confident. Moreover, the features obtained in both of these frameworks are observed to be highly consistent and generalizable. Additionally, we observe that the features relating the proteins to cell markers are especially important in automated learning approaches.",
keywords = "Feature selection, Image classification, Location proteomics, Machine learning, Microscopy",
author = "Newberg, {Justin Y.} and Jieyue Li and Arvind Rao and Fredrik Pont{\'e}n and Mathias Uhl{\'e}n and Emma Lundberg and Murphy, {Robert F.}",
year = "2009",
doi = "10.1109/ISBI.2009.5193229",
language = "English (US)",
isbn = "9781424439324",
series = "Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009",
pages = "1023--1026",
booktitle = "Proceedings - 2009 IEEE International Symposium on Biomedical Imaging",
note = "2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 ; Conference date: 28-06-2009 Through 01-07-2009",
}