TY - JOUR
T1 - B-lineage lymphoid blast crisis in juvenile chronic myelogenous leukemia
T2 - II interleukin-1-mediated autocrine growth regulation of the lymphoblasts
AU - Attias, D.
AU - Grunberger, T.
AU - Vanek, W.
AU - Estrov, Z.
AU - Cohen, A.
AU - Lau, R.
AU - Freedman, M. H.
PY - 1995/5
Y1 - 1995/5
N2 - A pre-B acute lymphoblastic leukemia (ALL) cell line with monosomy 7 was established from a child with juvenile chronic myelogenous leukemia (JCML) in lymphoid blast crisis. Analysis of the growth properties of the cell line, termed 'W1' showed an interleukin-1 (IL-1) mediated autocrine pattern of cell proliferation with the following features: W1 colony growth without added growth factor was density-dependent and colony growth was augmented with serum-free autologous cell culture supernatant; exogenous IL-1β had a growth-promoting effect on W1 colony numbers when cells were seeded at low density; W1 cells constitutively expressed mRNA for IL-1β, and high levels of IL-1β were measured in W1 cell lysates; anti-IL-1β antibodies as well as IL-1 receptor antagonist markedly suppressed W1 colony growth when either was added to cultures of cells seeded without growth factors at low density; anti-GM-CSF antibodies and anti-IL-3 antibodies had no inhibitory effect on W1 colony growth. Whereas W1 colony growth was also augmented by adding IL-3, IL-4, IL-6, IL-7, GM-CSF, Steel factor and erythropoietin individually to the cultures, W1 cells did not constitutively express mRNA for any of these cytokines. W1 colony growth was markedly suppressed by exogeneous TNF-α which contrasts sharply with the autocrine growth promoting effect of TNF-α on myelomonocytic elements of JCML in 'chronic' phase. The inhibitory effect of TNF-α on W1 cells was not due to downregulation of IL-1 production. The IL-1-dependent growth of W1 cells appeared to be unique because none of five other pre-B lineage ALL cell lines established as controls showed an autocrine growth loop via IL-1. W1 cells provide a valuable opportunity to examine the relationship of monosomy 7, B-lineage acute lymphoblastic leukemia, aberrant genetic expression of cytokines and their receptors, and IL-1 mediated autocrine cell growth in cancer.
AB - A pre-B acute lymphoblastic leukemia (ALL) cell line with monosomy 7 was established from a child with juvenile chronic myelogenous leukemia (JCML) in lymphoid blast crisis. Analysis of the growth properties of the cell line, termed 'W1' showed an interleukin-1 (IL-1) mediated autocrine pattern of cell proliferation with the following features: W1 colony growth without added growth factor was density-dependent and colony growth was augmented with serum-free autologous cell culture supernatant; exogenous IL-1β had a growth-promoting effect on W1 colony numbers when cells were seeded at low density; W1 cells constitutively expressed mRNA for IL-1β, and high levels of IL-1β were measured in W1 cell lysates; anti-IL-1β antibodies as well as IL-1 receptor antagonist markedly suppressed W1 colony growth when either was added to cultures of cells seeded without growth factors at low density; anti-GM-CSF antibodies and anti-IL-3 antibodies had no inhibitory effect on W1 colony growth. Whereas W1 colony growth was also augmented by adding IL-3, IL-4, IL-6, IL-7, GM-CSF, Steel factor and erythropoietin individually to the cultures, W1 cells did not constitutively express mRNA for any of these cytokines. W1 colony growth was markedly suppressed by exogeneous TNF-α which contrasts sharply with the autocrine growth promoting effect of TNF-α on myelomonocytic elements of JCML in 'chronic' phase. The inhibitory effect of TNF-α on W1 cells was not due to downregulation of IL-1 production. The IL-1-dependent growth of W1 cells appeared to be unique because none of five other pre-B lineage ALL cell lines established as controls showed an autocrine growth loop via IL-1. W1 cells provide a valuable opportunity to examine the relationship of monosomy 7, B-lineage acute lymphoblastic leukemia, aberrant genetic expression of cytokines and their receptors, and IL-1 mediated autocrine cell growth in cancer.
KW - Autocrine cell growth
KW - Interleukin-1
KW - Juvenile CML
KW - Lymphoid blast crisis
UR - http://www.scopus.com/inward/record.url?scp=0029034066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029034066&partnerID=8YFLogxK
M3 - Article
C2 - 7769852
AN - SCOPUS:0029034066
SN - 0887-6924
VL - 9
SP - 884
EP - 888
JO - Leukemia
JF - Leukemia
IS - 5
ER -