TY - JOUR
T1 - Backcrossing to Generate a Congenic Mouse Strain
AU - Papaioannou, Virginia E.
AU - Behringer, Richard R.
N1 - Publisher Copyright:
© 2024 Cold Spring Harbor Laboratory Press.
PY - 2024/1
Y1 - 2024/1
N2 - Genetic background can have subtle or profound effects on mutant phenotypes, providing additional information regarding the function of the gene. If your mutation is maintained on one genetic background but you wish to analyze it on another, it is a simple matter to transfer the mutation to a recipient strain background by repeated backcrossing (introgression) as detailed in this protocol. The resulting strain is called a congenic strain, defined as a strain carrying the mutation within a segment of chromosome from the donor strain with the remainder of the genome from the recipient strain.
AB - Genetic background can have subtle or profound effects on mutant phenotypes, providing additional information regarding the function of the gene. If your mutation is maintained on one genetic background but you wish to analyze it on another, it is a simple matter to transfer the mutation to a recipient strain background by repeated backcrossing (introgression) as detailed in this protocol. The resulting strain is called a congenic strain, defined as a strain carrying the mutation within a segment of chromosome from the donor strain with the remainder of the genome from the recipient strain.
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U2 - 10.1101/pdb.prot108039
DO - 10.1101/pdb.prot108039
M3 - Article
C2 - 37932101
AN - SCOPUS:85181585381
SN - 1940-3402
VL - 2024
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 1
ER -