TY - JOUR
T1 - Bacterial artificial chromosome array-Based comparative genomic hybridization using paired formalin-fixed, paraffin-embedded and fresh frozen tissue specimens in multiple myeloma
AU - Lennon, Patrick A.
AU - Zhuang, Yi
AU - Pierson, Diane
AU - Zhang, Xiang
AU - Williams, Chris
AU - Perez, Cintia
AU - Lin, Pei
PY - 2009/1/15
Y1 - 2009/1/15
N2 - BACKGROUND: Multiple myeloma (MM) is a neoplasm of malignant plasma cells that often harbors many chromosomal aberrations. Currently, fresh frozen tissues (FT) are considered the most reliable for molecular genetic analysis; however, formalin-fixed, paraffin-embedded (FFPE) tissues are easily retrievable. Compared with conventional cytogenetics, bacterial artificial chromosome (BAC) array-comparative genomic hybridization (CGH) allows more sensitive detection of chromosomal abnormalities. METHODS: The authors analyzed 7 paired FT and FFPE samples of bone marrow aspirate materials obtained from patients with MM in parallel to determine the efficacy of BAC array-CGH using FFPE. RESULTS: Thirty-four aberrations were identified, including 29 that were observed in both sample types, yielding 85% concordance. Nonran-dom anomalies, including gains on 7q, 9q, 15q, and 19p and losses on 8p and 13q, were observed in paired samples from at least 2 patients. To verify these results, fluorescence in situ hybridization (FISH) was performed using probes specific for 7q and 15q, and gains were observed in the 4 samples that were examined. Furthermore, 1 of 3 samples from patients who had monoclonal gammopathy of undetermined significance that were tested also carried gain on 7q, suggesting that this aberration may be an early transforming event. CONCLUSIONS: The current results indicated that BAC array-CGH can be effective using FFPE samples and is a sensitive method for the identification of nonrandom chromosomal aberrations in MM. Cancer 2009;115:345-54.
AB - BACKGROUND: Multiple myeloma (MM) is a neoplasm of malignant plasma cells that often harbors many chromosomal aberrations. Currently, fresh frozen tissues (FT) are considered the most reliable for molecular genetic analysis; however, formalin-fixed, paraffin-embedded (FFPE) tissues are easily retrievable. Compared with conventional cytogenetics, bacterial artificial chromosome (BAC) array-comparative genomic hybridization (CGH) allows more sensitive detection of chromosomal abnormalities. METHODS: The authors analyzed 7 paired FT and FFPE samples of bone marrow aspirate materials obtained from patients with MM in parallel to determine the efficacy of BAC array-CGH using FFPE. RESULTS: Thirty-four aberrations were identified, including 29 that were observed in both sample types, yielding 85% concordance. Nonran-dom anomalies, including gains on 7q, 9q, 15q, and 19p and losses on 8p and 13q, were observed in paired samples from at least 2 patients. To verify these results, fluorescence in situ hybridization (FISH) was performed using probes specific for 7q and 15q, and gains were observed in the 4 samples that were examined. Furthermore, 1 of 3 samples from patients who had monoclonal gammopathy of undetermined significance that were tested also carried gain on 7q, suggesting that this aberration may be an early transforming event. CONCLUSIONS: The current results indicated that BAC array-CGH can be effective using FFPE samples and is a sensitive method for the identification of nonrandom chromosomal aberrations in MM. Cancer 2009;115:345-54.
KW - Array comparative genomic hybridization
KW - Bacterial artificial chromosome
KW - Formalin-fixed
KW - Multiple myeloma
KW - Paraffin-embedded
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U2 - 10.1002/cncr.24021
DO - 10.1002/cncr.24021
M3 - Article
C2 - 19109814
AN - SCOPUS:59449090769
SN - 0008-543X
VL - 115
SP - 345
EP - 354
JO - Cancer
JF - Cancer
IS - 2
ER -