Bcl-2 inhibits p53 nuclear import following DNA damage

Alexander Beham; Maria C. Marin; Antonio Fernandez; John Herrmann; Shawn Brisbay; Ana M. Tari; Gabriel Lopez-Berestein; Guillermina Lozano; Mona Sarkiss; Timothy J. McDonnell

doi:10.1038/sj.onc.1201464

Bcl-2 inhibits p53 nuclear import following DNA damage

Alexander Beham, Maria C. Marin, Antonio Fernandez, John Herrmann, Shawn Brisbay, Ana M. Tari, Gabriel Lopez-Berestein, Guillermina Lozano, Mona Sarkiss, Timothy J. McDonnell

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

Bcl-2 is an integral membrane oncoprotein that localizes to membranes of the mitochondria, endoplasmic reticulum, and nuclear envelope. Bcl-2 is a member of a family of cell death regulators and functions to inhibit apoptosis. Using confocal microscopy and immunoblotting we show that the ability of bcl-2 to suppress cell death following genotoxic damage can be a consequence of inhibiting nuclear import of induced wild-type p53 protein. Our data suggests that the ability of bcl-2 to modulate trafficking events is not cell type specific. These data support a 'gatekeeper' mechanism for cell death suppression by bcl-2.

Original language	English (US)
Pages (from-to)	2767-2772
Number of pages	6
Journal	Oncogene
Volume	15
Issue number	23
DOIs	https://doi.org/10.1038/sj.onc.1201464
State	Published - 1997

Keywords

Apoptosis
Bcl-2
Nuclear import
Prostate cancer
p53

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1038/sj.onc.1201464

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title = "Bcl-2 inhibits p53 nuclear import following DNA damage",

abstract = "Bcl-2 is an integral membrane oncoprotein that localizes to membranes of the mitochondria, endoplasmic reticulum, and nuclear envelope. Bcl-2 is a member of a family of cell death regulators and functions to inhibit apoptosis. Using confocal microscopy and immunoblotting we show that the ability of bcl-2 to suppress cell death following genotoxic damage can be a consequence of inhibiting nuclear import of induced wild-type p53 protein. Our data suggests that the ability of bcl-2 to modulate trafficking events is not cell type specific. These data support a 'gatekeeper' mechanism for cell death suppression by bcl-2.",

keywords = "Apoptosis, Bcl-2, Nuclear import, Prostate cancer, p53",

author = "Alexander Beham and Marin, {Maria C.} and Antonio Fernandez and John Herrmann and Shawn Brisbay and Tari, {Ana M.} and Gabriel Lopez-Berestein and Guillermina Lozano and Mona Sarkiss and McDonnell, {Timothy J.}",

note = "Funding Information: Supported by ACS grant DHP-156 and CaP CURE (The Association for the Cure of Cancer of the Prostate) and the Pew Scholars Program in the Biomedical Sciences. MCM",

year = "1997",

doi = "10.1038/sj.onc.1201464",

language = "English (US)",

volume = "15",

pages = "2767--2772",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Nature Publishing Group",

number = "23",

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TY - JOUR

T1 - Bcl-2 inhibits p53 nuclear import following DNA damage

AU - Beham, Alexander

AU - Marin, Maria C.

AU - Fernandez, Antonio

AU - Herrmann, John

AU - Brisbay, Shawn

AU - Tari, Ana M.

AU - Lopez-Berestein, Gabriel

AU - Lozano, Guillermina

AU - Sarkiss, Mona

AU - McDonnell, Timothy J.

N1 - Funding Information: Supported by ACS grant DHP-156 and CaP CURE (The Association for the Cure of Cancer of the Prostate) and the Pew Scholars Program in the Biomedical Sciences. MCM

PY - 1997

Y1 - 1997

N2 - Bcl-2 is an integral membrane oncoprotein that localizes to membranes of the mitochondria, endoplasmic reticulum, and nuclear envelope. Bcl-2 is a member of a family of cell death regulators and functions to inhibit apoptosis. Using confocal microscopy and immunoblotting we show that the ability of bcl-2 to suppress cell death following genotoxic damage can be a consequence of inhibiting nuclear import of induced wild-type p53 protein. Our data suggests that the ability of bcl-2 to modulate trafficking events is not cell type specific. These data support a 'gatekeeper' mechanism for cell death suppression by bcl-2.

AB - Bcl-2 is an integral membrane oncoprotein that localizes to membranes of the mitochondria, endoplasmic reticulum, and nuclear envelope. Bcl-2 is a member of a family of cell death regulators and functions to inhibit apoptosis. Using confocal microscopy and immunoblotting we show that the ability of bcl-2 to suppress cell death following genotoxic damage can be a consequence of inhibiting nuclear import of induced wild-type p53 protein. Our data suggests that the ability of bcl-2 to modulate trafficking events is not cell type specific. These data support a 'gatekeeper' mechanism for cell death suppression by bcl-2.

KW - Apoptosis

KW - Bcl-2

KW - Nuclear import

KW - Prostate cancer

KW - p53

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DO - 10.1038/sj.onc.1201464

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SN - 0950-9232

VL - 15

SP - 2767

EP - 2772

JO - Oncogene

JF - Oncogene

IS - 23

ER -

Bcl-2 inhibits p53 nuclear import following DNA damage

Abstract

Keywords

ASJC Scopus subject areas

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