TY - JOUR
T1 - Biodistribution and PET imaging of [18F]-fluoroadenosine derivatives
AU - Alauddin, Mian M.
AU - Shahinian, Antranik
AU - Park, Ryan
AU - Tohme, Michael
AU - Fissekis, John D.
AU - Conti, Peter S.
N1 - Funding Information:
This work was supported by the National Cancer Institute Grant CA 72896.
PY - 2007/4
Y1 - 2007/4
N2 - Introduction: Many fluorinated analogues of adenosine nucleoside have been synthesized and studied as potential antitumor and antiviral agents. Earlier, we reported radiosynthesis of 2′-deoxy-2′-[18F]fluoro-1-β-d-arabinofuranosyl-adenine ([18F]-FAA) and 3′-deoxy-3′-[18F]fluoro-1-β-d-xylofuranosyl-adenine ([18F]FXA). Now, we report their in vivo studies including blood clearance, biodistribution and micro-PET imaging in tumor-bearing nude mice. Methods: Tumors were grown in 6-week-old athymic nude mice (Harlan, Indianapolis, IN, USA) by inoculation of HT-29 cells, wild-type cells in the left flank and transduced cells with HSV-tk on the right flank. When the tumor was about 1 cm in size, animals were injected with these radiotracers for in vivo studies, including blood clearance, micro-PET imaging and biodistribution. Results: Uptake of [18F]FAA in tumor was 3.3-fold higher than blood, with highest uptake in the spleen. Maximum uptake of [18F]FXA was observed in the heart compared to other organs. There was no tumor uptake of [18F]FXA. Biodistribution results were supported by micro-PET images, which also showed very high uptake of [18F]FAA in spleen and visualization of tumors, and high uptake of [18F]FXA in the heart. Conclusion: These results suggest that [18F]FAA may be useful for tumor imaging, while [18F]FXA may have potential as a heart imaging agent with PET.
AB - Introduction: Many fluorinated analogues of adenosine nucleoside have been synthesized and studied as potential antitumor and antiviral agents. Earlier, we reported radiosynthesis of 2′-deoxy-2′-[18F]fluoro-1-β-d-arabinofuranosyl-adenine ([18F]-FAA) and 3′-deoxy-3′-[18F]fluoro-1-β-d-xylofuranosyl-adenine ([18F]FXA). Now, we report their in vivo studies including blood clearance, biodistribution and micro-PET imaging in tumor-bearing nude mice. Methods: Tumors were grown in 6-week-old athymic nude mice (Harlan, Indianapolis, IN, USA) by inoculation of HT-29 cells, wild-type cells in the left flank and transduced cells with HSV-tk on the right flank. When the tumor was about 1 cm in size, animals were injected with these radiotracers for in vivo studies, including blood clearance, micro-PET imaging and biodistribution. Results: Uptake of [18F]FAA in tumor was 3.3-fold higher than blood, with highest uptake in the spleen. Maximum uptake of [18F]FXA was observed in the heart compared to other organs. There was no tumor uptake of [18F]FXA. Biodistribution results were supported by micro-PET images, which also showed very high uptake of [18F]FAA in spleen and visualization of tumors, and high uptake of [18F]FXA in the heart. Conclusion: These results suggest that [18F]FAA may be useful for tumor imaging, while [18F]FXA may have potential as a heart imaging agent with PET.
KW - Adenosine
KW - Micro-PET
KW - [F]-FAA
KW - [F]-FXA
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U2 - 10.1016/j.nucmedbio.2006.12.009
DO - 10.1016/j.nucmedbio.2006.12.009
M3 - Article
C2 - 17383576
AN - SCOPUS:33947305259
SN - 0969-8051
VL - 34
SP - 267
EP - 272
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
IS - 3
ER -