Biological responses of human solid tumor cells to X-ray irradiation within a 1.5-Tesla magnetic field generated by a magnetic resonance imaging-linear accelerator

Li Wang; Stan Jelle Hoogcarspel; Zhifei Wen; Marco van Vulpen; David P. Molkentine; Jan Kok; Steven H. Lin; Roel Broekhuizen; Kie Kian Ang; Niels Bovenschen; Bas W. Raaymakers; Steven J. Frank

doi:10.1002/bem.21991

Biological responses of human solid tumor cells to X-ray irradiation within a 1.5-Tesla magnetic field generated by a magnetic resonance imaging-linear accelerator

Li Wang, Stan Jelle Hoogcarspel, Zhifei Wen, Marco van Vulpen, David P. Molkentine, Jan Kok, Steven H. Lin, Roel Broekhuizen, Kie Kian Ang, Niels Bovenschen, Bas W. Raaymakers, Steven J. Frank

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Devices that combine magnetic resonance imaging with linear accelerators (MRL) represent a novel tool for MR-guided radiotherapy. However, whether magnetic fields (MFs) generated by these devices affect the radiosensitivity of tumors is unknown. We investigated the influence of a 1.5-T MF on cell viability and radioresponse of human solid tumors. Human head/neck cancer and lung cancer cells were exposed to single or fractionated 6-MV X-ray radiation; effects of the MF on cell viability were determined by cell plating efficiency and on radioresponsiveness by clonogenic cell survival. Doses needed to reduce the fraction of surviving cells to 37% of the initial value (D0s) were calculated for multiple exposures to MF and radiation. Results were analyzed using Student's t-tests. Cell viability was no different after single or multiple exposures to MRL than after exposure to a conventional linear accelerator (Linac, without MR-generated MF) in 12 of 15 experiments (all P > 0.05). Single or multiple exposures to MF had no influence on cell radioresponse (all P > 0.05). Cells treated up to four times with an MRL or a Linac further showed no changes in D0s with MF versus without MF (all P > 0.05). In conclusion, MF within the MRL does not seem to affect in vitro tumor radioresponsiveness as compared with a conventional Linac. Bioelectromagnetics. 37:471-480, 2016.

Original language	English (US)
Pages (from-to)	471-480
Number of pages	10
Journal	Bioelectromagnetics
Volume	37
Issue number	7
DOIs	https://doi.org/10.1002/bem.21991
State	Published - Oct 1 2016

Keywords

MRI-linear accelerator
cell radioresponse
cell viability
head and neck cancer cells
lung cancer cells

ASJC Scopus subject areas

Biophysics
Physiology
Radiology Nuclear Medicine and imaging

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10.1002/bem.21991

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Wang, L., Hoogcarspel, S. J., Wen, Z., van Vulpen, M., Molkentine, D. P., Kok, J., Lin, S. H., Broekhuizen, R., Ang, K. K., Bovenschen, N., Raaymakers, B. W., & Frank, S. J. (2016). Biological responses of human solid tumor cells to X-ray irradiation within a 1.5-Tesla magnetic field generated by a magnetic resonance imaging-linear accelerator. Bioelectromagnetics, 37(7), 471-480. https://doi.org/10.1002/bem.21991

Wang, L, Hoogcarspel, SJ, Wen, Z, van Vulpen, M, Molkentine, DP, Kok, J, Lin, SH, Broekhuizen, R, Ang, KK, Bovenschen, N, Raaymakers, BW & Frank, SJ 2016, 'Biological responses of human solid tumor cells to X-ray irradiation within a 1.5-Tesla magnetic field generated by a magnetic resonance imaging-linear accelerator', Bioelectromagnetics, vol. 37, no. 7, pp. 471-480. https://doi.org/10.1002/bem.21991

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title = "Biological responses of human solid tumor cells to X-ray irradiation within a 1.5-Tesla magnetic field generated by a magnetic resonance imaging-linear accelerator",

abstract = "Devices that combine magnetic resonance imaging with linear accelerators (MRL) represent a novel tool for MR-guided radiotherapy. However, whether magnetic fields (MFs) generated by these devices affect the radiosensitivity of tumors is unknown. We investigated the influence of a 1.5-T MF on cell viability and radioresponse of human solid tumors. Human head/neck cancer and lung cancer cells were exposed to single or fractionated 6-MV X-ray radiation; effects of the MF on cell viability were determined by cell plating efficiency and on radioresponsiveness by clonogenic cell survival. Doses needed to reduce the fraction of surviving cells to 37% of the initial value (D0s) were calculated for multiple exposures to MF and radiation. Results were analyzed using Student's t-tests. Cell viability was no different after single or multiple exposures to MRL than after exposure to a conventional linear accelerator (Linac, without MR-generated MF) in 12 of 15 experiments (all P > 0.05). Single or multiple exposures to MF had no influence on cell radioresponse (all P > 0.05). Cells treated up to four times with an MRL or a Linac further showed no changes in D0s with MF versus without MF (all P > 0.05). In conclusion, MF within the MRL does not seem to affect in vitro tumor radioresponsiveness as compared with a conventional Linac. Bioelectromagnetics. 37:471-480, 2016.",

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AU - Wang, Li

AU - Hoogcarspel, Stan Jelle

AU - Wen, Zhifei

AU - van Vulpen, Marco

AU - Molkentine, David P.

AU - Kok, Jan

AU - Lin, Steven H.

AU - Broekhuizen, Roel

AU - Ang, Kie Kian

AU - Bovenschen, Niels

AU - Raaymakers, Bas W.

AU - Frank, Steven J.

PY - 2016/10/1

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N2 - Devices that combine magnetic resonance imaging with linear accelerators (MRL) represent a novel tool for MR-guided radiotherapy. However, whether magnetic fields (MFs) generated by these devices affect the radiosensitivity of tumors is unknown. We investigated the influence of a 1.5-T MF on cell viability and radioresponse of human solid tumors. Human head/neck cancer and lung cancer cells were exposed to single or fractionated 6-MV X-ray radiation; effects of the MF on cell viability were determined by cell plating efficiency and on radioresponsiveness by clonogenic cell survival. Doses needed to reduce the fraction of surviving cells to 37% of the initial value (D0s) were calculated for multiple exposures to MF and radiation. Results were analyzed using Student's t-tests. Cell viability was no different after single or multiple exposures to MRL than after exposure to a conventional linear accelerator (Linac, without MR-generated MF) in 12 of 15 experiments (all P > 0.05). Single or multiple exposures to MF had no influence on cell radioresponse (all P > 0.05). Cells treated up to four times with an MRL or a Linac further showed no changes in D0s with MF versus without MF (all P > 0.05). In conclusion, MF within the MRL does not seem to affect in vitro tumor radioresponsiveness as compared with a conventional Linac. Bioelectromagnetics. 37:471-480, 2016.

AB - Devices that combine magnetic resonance imaging with linear accelerators (MRL) represent a novel tool for MR-guided radiotherapy. However, whether magnetic fields (MFs) generated by these devices affect the radiosensitivity of tumors is unknown. We investigated the influence of a 1.5-T MF on cell viability and radioresponse of human solid tumors. Human head/neck cancer and lung cancer cells were exposed to single or fractionated 6-MV X-ray radiation; effects of the MF on cell viability were determined by cell plating efficiency and on radioresponsiveness by clonogenic cell survival. Doses needed to reduce the fraction of surviving cells to 37% of the initial value (D0s) were calculated for multiple exposures to MF and radiation. Results were analyzed using Student's t-tests. Cell viability was no different after single or multiple exposures to MRL than after exposure to a conventional linear accelerator (Linac, without MR-generated MF) in 12 of 15 experiments (all P > 0.05). Single or multiple exposures to MF had no influence on cell radioresponse (all P > 0.05). Cells treated up to four times with an MRL or a Linac further showed no changes in D0s with MF versus without MF (all P > 0.05). In conclusion, MF within the MRL does not seem to affect in vitro tumor radioresponsiveness as compared with a conventional Linac. Bioelectromagnetics. 37:471-480, 2016.

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Biological responses of human solid tumor cells to X-ray irradiation within a 1.5-Tesla magnetic field generated by a magnetic resonance imaging-linear accelerator

Abstract

Keywords

ASJC Scopus subject areas

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