TY - JOUR
T1 - Biosynthesis of colony-stimulating factor-1 (CSF-1) by mesangial cells of autoimmune mice
AU - Mishra, L.
AU - Ooi, B. S.
N1 - Funding Information:
The research was supported in part by a grant from the Department of Veterans Affairs. The excellent technical assistance of Y.J. Liu and A. Saini is gratefully acknowledged as is the secretarial assistance of Denise Suggs.
PY - 1993
Y1 - 1993
N2 - The amounts of colony-stimulating factor (CSF-1) produced by the mesangial cells of the kidney may be of pivotal importance in determining the outcome of nephritis, since CSF-1 activates macrophages, and macrophages have been shown to perform an important scavenger function in removing immune complexes which localize in the kidney. To test this hypothesis, the present study examined CSF-1 production by mesangial cells of the autoimmune MRL/MpJ-Ipr-Ipr strain of mice and also of their normal congenic MRL/MpJ -++ counterparts. It was found that mesangial cells of autoimmune mice produced diminished amounts of functional CSF-1 when measured by a bioassay, giving validation to the hypothesis proposed. Of interest, expression of CSF-1 mRNA was discordantly increased in mesangial cells of the MRL-Ipr autoimmune mice. Based on current knowledge of the pathway for CSF-1 synthesis and secretion, it would appear that there may be a block in the post-transcriptional pathway for CSF-1 biosynthesis in autoimmune mesangial cells.
AB - The amounts of colony-stimulating factor (CSF-1) produced by the mesangial cells of the kidney may be of pivotal importance in determining the outcome of nephritis, since CSF-1 activates macrophages, and macrophages have been shown to perform an important scavenger function in removing immune complexes which localize in the kidney. To test this hypothesis, the present study examined CSF-1 production by mesangial cells of the autoimmune MRL/MpJ-Ipr-Ipr strain of mice and also of their normal congenic MRL/MpJ -++ counterparts. It was found that mesangial cells of autoimmune mice produced diminished amounts of functional CSF-1 when measured by a bioassay, giving validation to the hypothesis proposed. Of interest, expression of CSF-1 mRNA was discordantly increased in mesangial cells of the MRL-Ipr autoimmune mice. Based on current knowledge of the pathway for CSF-1 synthesis and secretion, it would appear that there may be a block in the post-transcriptional pathway for CSF-1 biosynthesis in autoimmune mesangial cells.
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U2 - 10.3109/08820139309063406
DO - 10.3109/08820139309063406
M3 - Article
C2 - 8509159
AN - SCOPUS:0027537978
SN - 0882-0139
VL - 22
SP - 249
EP - 255
JO - Immunological Investigations
JF - Immunological Investigations
IS - 3
ER -