Abstract
Cancer stem cells can survive and undergo transformation after apoptosis by initiating robust endocytosis. Endocytosis in-turn drives formation of serpentine filopodia, which promote construction of blebbishields from apoptotic bodies. However, the status and role of macropinocytosis in blebbishields is not known. Here, we show by scanning electron microscopy and by macropinocytosis assays that blebbishields exhibit robust macropinocytosis. Inhibiting dynamin-mediated endocytosis does not affect macropinocytosis in blebbishields or in mitotic cells. In addition, inhibiting macropinocytosis did not inhibit construction of blebbishields from apoptotic bodies. Thus, although apoptotic cancer stem cells exhibit robust macropinocytosis, macropinocytosis is not essential to generate blebbishields, although it may play other roles in blebbishield biology.
Original language | English (US) |
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Pages (from-to) | 181-186 |
Number of pages | 6 |
Journal | BioFactors |
Volume | 43 |
Issue number | 2 |
DOIs | |
State | Published - Mar 1 2017 |
Keywords
- Smac mimetic
- apoptosis
- dynamin
- macropinocytosis
- phagocytic cup
- transformation
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Clinical Biochemistry
MD Anderson CCSG core facilities
- High Resolution Electron Microscopy Facility