BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells

James P. Vaughn; Penelope L. Davis; Marcus D. Jarboe; Gudrun Huper; A. Craig Evans; Roger W. Wiseman; Andrew Berchuck; J. Dirk Iglehart; P. Andrew Futreal; Jeffrey R. Marks

BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells

James P. Vaughn, Penelope L. Davis, Marcus D. Jarboe, Gudrun Huper, A. Craig Evans, Roger W. Wiseman, Andrew Berchuck, J. Dirk Iglehart, P. Andrew Futreal, Jeffrey R. Marks

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

Insight into the function of the BRCA1 tumor suppressor gene may be gained by studying its regulation. In this study, the expression of BRCA1 was examined as a function of the cell cycle in normal and tumor-derived breast epithelial cells. Cells arrested in G₀ or early in G₁ contained low levels of BRCA1 mRNA. After release, populations of cells reached maximal levels of BRCA1 in late G₁ and S phase. Induction of BRCA1 was shown to occur before the onset of DNA synthesis by synchronizing cells at the G₁-S boundary. Levels of the BRCA1 protein were regulated in a similar manner. No difference was observed between primary cultures of normal mammary epithelial cells and immortalized tumor-derived cell lines. These results suggest that BRCA1 may function at the G₁-S checkpoint.

Original language	English (US)
Pages (from-to)	711-715
Number of pages	5
Journal	Cell Growth and Differentiation
Volume	7
Issue number	6
State	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Cite this

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title = "BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells",

abstract = "Insight into the function of the BRCA1 tumor suppressor gene may be gained by studying its regulation. In this study, the expression of BRCA1 was examined as a function of the cell cycle in normal and tumor-derived breast epithelial cells. Cells arrested in G0 or early in G1 contained low levels of BRCA1 mRNA. After release, populations of cells reached maximal levels of BRCA1 in late G1 and S phase. Induction of BRCA1 was shown to occur before the onset of DNA synthesis by synchronizing cells at the G1-S boundary. Levels of the BRCA1 protein were regulated in a similar manner. No difference was observed between primary cultures of normal mammary epithelial cells and immortalized tumor-derived cell lines. These results suggest that BRCA1 may function at the G1-S checkpoint.",

author = "Vaughn, {James P.} and Davis, {Penelope L.} and Jarboe, {Marcus D.} and Gudrun Huper and {Craig Evans}, A. and Wiseman, {Roger W.} and Andrew Berchuck and Iglehart, {J. Dirk} and Futreal, {P. Andrew} and Marks, {Jeffrey R.}",

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T1 - BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells

AU - Vaughn, James P.

AU - Davis, Penelope L.

AU - Jarboe, Marcus D.

AU - Huper, Gudrun

AU - Craig Evans, A.

AU - Wiseman, Roger W.

AU - Berchuck, Andrew

AU - Iglehart, J. Dirk

AU - Futreal, P. Andrew

AU - Marks, Jeffrey R.

PY - 1996

Y1 - 1996

N2 - Insight into the function of the BRCA1 tumor suppressor gene may be gained by studying its regulation. In this study, the expression of BRCA1 was examined as a function of the cell cycle in normal and tumor-derived breast epithelial cells. Cells arrested in G0 or early in G1 contained low levels of BRCA1 mRNA. After release, populations of cells reached maximal levels of BRCA1 in late G1 and S phase. Induction of BRCA1 was shown to occur before the onset of DNA synthesis by synchronizing cells at the G1-S boundary. Levels of the BRCA1 protein were regulated in a similar manner. No difference was observed between primary cultures of normal mammary epithelial cells and immortalized tumor-derived cell lines. These results suggest that BRCA1 may function at the G1-S checkpoint.

AB - Insight into the function of the BRCA1 tumor suppressor gene may be gained by studying its regulation. In this study, the expression of BRCA1 was examined as a function of the cell cycle in normal and tumor-derived breast epithelial cells. Cells arrested in G0 or early in G1 contained low levels of BRCA1 mRNA. After release, populations of cells reached maximal levels of BRCA1 in late G1 and S phase. Induction of BRCA1 was shown to occur before the onset of DNA synthesis by synchronizing cells at the G1-S boundary. Levels of the BRCA1 protein were regulated in a similar manner. No difference was observed between primary cultures of normal mammary epithelial cells and immortalized tumor-derived cell lines. These results suggest that BRCA1 may function at the G1-S checkpoint.

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BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells

Abstract

ASJC Scopus subject areas

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