@article{dcfffe61c0e945f7895cfeb8693c5b1d,
title = "Caging proteins through unnatural amino acid mutagenesis",
abstract = "The caging of specific residues of proteins is a powerful tool. This discussion attempts to alert the reader to the considerations that must be made in preparing and analyzing a caged protein through nonsense suppression. Although the suppression methodology is conceptually straightforward, it not possible to provide a failsafe {"}cook book{"} method for using caged unnaturals. We have emphasized the preparation of caged receptors expressed in Xenopus oocytes, but these approaches can clearly be adapted to many other systems.",
author = "Petersson, {E. James} and Brandt, {Gabriel S.} and Zacharias, {Niki M.} and Dougherty, {Dennis A.} and Lester, {Henry A.}",
note = "Funding Information: This work was supported by the National Institutes of Health (Grants GM-29836, NS-11756, NS-34407, and NS-11756). Funding Information: We thank Cynthia Earles, Ward Tucker, and Ralf Langen for critical comments on this manuscript and David Gaston for molecular modeling. This study was supported by grants from the NIH (GM 56827-01 and AHA 9750326N), and by the Milwaukee Foundation. E.R.C. is a Pew Scholar in the Biomedical Sciences. J.B. is supported by an AHA Pre-doctoral Fellowship.",
year = "2003",
month = jan,
day = "1",
doi = "10.1016/S0076-6879(03)60114-X",
language = "English (US)",
volume = "360",
pages = "258--273",
journal = "Methods in enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
}