Abstract
The pre-mRNA encoding calcitonin (CT) and CT gene-related peptide (CGRP) is differentially processed in a tissue-specific fashion to include exon 4 (which encodes CT) or exclude this exon and splice to exon 5 (which encodes CGRP). We have used a CT-specific in vitro RNA-processing system to identify cis-acting sequences required to prevent splicing to exon 5. Deletion mapping demonstrated the presence of an element within the first 45 nucleotides of the CT-specific exon 4 that was required to suppress splicing to the CGRP-specific exon 5. This element was able to function in a completely heterologous system to suppress splicing when the CGRP exon was replaced with a constitutive viral exon. The element was unable to suppress splicing in the absence of a proximal CT-specific 3′ splice site. Our results suggest that CT-specific splicing requires assisted recognition of its 3′ splice site.
Original language | English (US) |
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Pages (from-to) | 1744-1749 |
Number of pages | 6 |
Journal | Molecular Endocrinology |
Volume | 4 |
Issue number | 11 |
State | Published - 1990 |
ASJC Scopus subject areas
- Molecular Biology
- Endocrinology