Calcium-activated DNA fragmentation in rat liver nuclei

D. P. Jones; D. J. McConkey; P. Nicotera; S. Orrenius

Calcium-activated DNA fragmentation in rat liver nuclei

D. P. Jones, D. J. McConkey, P. Nicotera, S. Orrenius

Urology

Research output: Contribution to journal › Article › peer-review

287 Scopus citations

Abstract

Incubation of isolated rat liver nuclei with ATP, NAD⁺, and submicromolar Ca²⁺ concentrations resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 200 nM Ca²⁺, and saturation of the process was observed with 1 μM Ca²⁺. ATP stimulated a calmodulin-dependent nuclear Ca²⁺ uptake system which apparently mediated endonuclease activation. Ca²⁺-activated DNA fragmentation was inhibited by the inhibitor of poly(ADP-ribose) synthetase, 3-aminobenzamide, and was associated with poly(ADP-ribosyl)ation of nuclear protein. The characteristics of this endonuclease activity indicate that it may be responsible for the Ca²⁺-dependent fragmentation of DNA involved in programmed cell death (apoptosis) and in certain forms of chemically induced cell killing.

Original language	English (US)
Pages (from-to)	6398-6403
Number of pages	6
Journal	Journal of Biological Chemistry
Volume	264
Issue number	11
State	Published - 1989

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

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title = "Calcium-activated DNA fragmentation in rat liver nuclei",

abstract = "Incubation of isolated rat liver nuclei with ATP, NAD+, and submicromolar Ca2+ concentrations resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 200 nM Ca2+, and saturation of the process was observed with 1 μM Ca2+. ATP stimulated a calmodulin-dependent nuclear Ca2+ uptake system which apparently mediated endonuclease activation. Ca2+-activated DNA fragmentation was inhibited by the inhibitor of poly(ADP-ribose) synthetase, 3-aminobenzamide, and was associated with poly(ADP-ribosyl)ation of nuclear protein. The characteristics of this endonuclease activity indicate that it may be responsible for the Ca2+-dependent fragmentation of DNA involved in programmed cell death (apoptosis) and in certain forms of chemically induced cell killing.",

author = "Jones, {D. P.} and McConkey, {D. J.} and P. Nicotera and S. Orrenius",

year = "1989",

language = "English (US)",

volume = "264",

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journal = "Journal of Biological Chemistry",

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T1 - Calcium-activated DNA fragmentation in rat liver nuclei

AU - Jones, D. P.

AU - McConkey, D. J.

AU - Nicotera, P.

AU - Orrenius, S.

PY - 1989

Y1 - 1989

N2 - Incubation of isolated rat liver nuclei with ATP, NAD+, and submicromolar Ca2+ concentrations resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 200 nM Ca2+, and saturation of the process was observed with 1 μM Ca2+. ATP stimulated a calmodulin-dependent nuclear Ca2+ uptake system which apparently mediated endonuclease activation. Ca2+-activated DNA fragmentation was inhibited by the inhibitor of poly(ADP-ribose) synthetase, 3-aminobenzamide, and was associated with poly(ADP-ribosyl)ation of nuclear protein. The characteristics of this endonuclease activity indicate that it may be responsible for the Ca2+-dependent fragmentation of DNA involved in programmed cell death (apoptosis) and in certain forms of chemically induced cell killing.

AB - Incubation of isolated rat liver nuclei with ATP, NAD+, and submicromolar Ca2+ concentrations resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 200 nM Ca2+, and saturation of the process was observed with 1 μM Ca2+. ATP stimulated a calmodulin-dependent nuclear Ca2+ uptake system which apparently mediated endonuclease activation. Ca2+-activated DNA fragmentation was inhibited by the inhibitor of poly(ADP-ribose) synthetase, 3-aminobenzamide, and was associated with poly(ADP-ribosyl)ation of nuclear protein. The characteristics of this endonuclease activity indicate that it may be responsible for the Ca2+-dependent fragmentation of DNA involved in programmed cell death (apoptosis) and in certain forms of chemically induced cell killing.

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M3 - Article

C2 - 2703497

AN - SCOPUS:0024543754

SN - 0021-9258

VL - 264

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 11

ER -

Calcium-activated DNA fragmentation in rat liver nuclei

Abstract

ASJC Scopus subject areas

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