TY - JOUR
T1 - Can cancer cells transform normal host cells into malignant cells?
AU - Pathak, S.
AU - Nemeth, M. A.
AU - Multani, A. S.
AU - Thalmann, G. N.
AU - Von Eschenbach, A. C.
AU - Chung, L. W.K.
N1 - Funding Information:
This work was supported in part by the National Institute of Health grants CA 64863 and RRO-4999-01 and a grant from the John S Dunn Research Foundation of Houston, Texas, USA. We thank Joyce E Benjamin for secretarial assistance and Leslie Wildrick for editorial help.
PY - 1997
Y1 - 1997
N2 - A human prostate tumour cell line, LNCaP C4-2, when injected into athymic male nude mice, produced tumours containing: (1) only human cancer cells similar to those injected; (2) only murine stromal cells containing abnormal chromosome constitutions; or (3) both human prostate cancer cells similar to those injected and the transformed murine stromal cells with altered chromosome constitutions. Karyotypic analysis of murine metaphases from all the host-derived tumours showed mostly pseudodiploid chromosome constitutions, with multiple copies (amplification) of mouse chromosome 15 and the absence of a typical Y chromosome. Fluorescence in situ hybridization analysis of these murine cells, using a biotin-labelled total human DNA painting probe, further demonstrated the absence of human DNA and the presence of only mouse metaphase and interphase cells in these transformed stromal cells. These results suggest that cancer cells are capable of inducing neoplastic transformation in stromal cells of the host organ by some, as yet unknown, epigenetic mechanism(s).
AB - A human prostate tumour cell line, LNCaP C4-2, when injected into athymic male nude mice, produced tumours containing: (1) only human cancer cells similar to those injected; (2) only murine stromal cells containing abnormal chromosome constitutions; or (3) both human prostate cancer cells similar to those injected and the transformed murine stromal cells with altered chromosome constitutions. Karyotypic analysis of murine metaphases from all the host-derived tumours showed mostly pseudodiploid chromosome constitutions, with multiple copies (amplification) of mouse chromosome 15 and the absence of a typical Y chromosome. Fluorescence in situ hybridization analysis of these murine cells, using a biotin-labelled total human DNA painting probe, further demonstrated the absence of human DNA and the presence of only mouse metaphase and interphase cells in these transformed stromal cells. These results suggest that cancer cells are capable of inducing neoplastic transformation in stromal cells of the host organ by some, as yet unknown, epigenetic mechanism(s).
KW - Epigenetic mechanism of carcinogenesis
KW - Fluorescence in situ hybridization
KW - Malignant transformation
KW - Prostate cancer progression
KW - Pseudodiploid karyotype
KW - Tumour-stroma interaction
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U2 - 10.1038/bjc.1997.524
DO - 10.1038/bjc.1997.524
M3 - Article
C2 - 9365160
AN - SCOPUS:0030799935
SN - 0007-0920
VL - 76
SP - 1134
EP - 1138
JO - British journal of cancer
JF - British journal of cancer
IS - 9
ER -