Cancer and genomics

P. A. Futreal; A. Kasprzyk; E. Birney; J. C. Mullikin; R. Wooster; M. R. Stratton

doi:10.1038/35057046

Cancer and genomics

P. A. Futreal, A. Kasprzyk, E. Birney, J. C. Mullikin, R. Wooster, M. R. Stratton

Research output: Contribution to journal › Review article › peer-review

132 Scopus citations

Abstract

Identification of the genes that cause oncogenesis is a central aim of cancer research. We searched the proteins predicted from the draft human genome sequence for paralogues of known tumour suppressor genes, but no novel genes were identified. We then assessed whether it was possible to search directly for oncogenic sequence changes in cancer cells by comparing cancer genome sequences against the draft genome. Apparently chimaeric transcripts (from oncogenic fusion genes generated by chromosomal translocations, the ends of which mapped to different genomic locations) were detected to the same degree in both normal and neoplastic tissues, indicating a significant level of false positives. Our experiment underscores the limited amount and variable quality of DNA sequence from cancer cells that is currently available.

Original language	English (US)
Pages (from-to)	850-852
Number of pages	3
Journal	Nature
Volume	409
Issue number	6822
DOIs	https://doi.org/10.1038/35057046
State	Published - Feb 15 2001
Externally published	Yes

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title = "Cancer and genomics",

abstract = "Identification of the genes that cause oncogenesis is a central aim of cancer research. We searched the proteins predicted from the draft human genome sequence for paralogues of known tumour suppressor genes, but no novel genes were identified. We then assessed whether it was possible to search directly for oncogenic sequence changes in cancer cells by comparing cancer genome sequences against the draft genome. Apparently chimaeric transcripts (from oncogenic fusion genes generated by chromosomal translocations, the ends of which mapped to different genomic locations) were detected to the same degree in both normal and neoplastic tissues, indicating a significant level of false positives. Our experiment underscores the limited amount and variable quality of DNA sequence from cancer cells that is currently available.",

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T1 - Cancer and genomics

AU - Futreal, P. A.

AU - Kasprzyk, A.

AU - Birney, E.

AU - Mullikin, J. C.

AU - Wooster, R.

AU - Stratton, M. R.

PY - 2001/2/15

Y1 - 2001/2/15

N2 - Identification of the genes that cause oncogenesis is a central aim of cancer research. We searched the proteins predicted from the draft human genome sequence for paralogues of known tumour suppressor genes, but no novel genes were identified. We then assessed whether it was possible to search directly for oncogenic sequence changes in cancer cells by comparing cancer genome sequences against the draft genome. Apparently chimaeric transcripts (from oncogenic fusion genes generated by chromosomal translocations, the ends of which mapped to different genomic locations) were detected to the same degree in both normal and neoplastic tissues, indicating a significant level of false positives. Our experiment underscores the limited amount and variable quality of DNA sequence from cancer cells that is currently available.

AB - Identification of the genes that cause oncogenesis is a central aim of cancer research. We searched the proteins predicted from the draft human genome sequence for paralogues of known tumour suppressor genes, but no novel genes were identified. We then assessed whether it was possible to search directly for oncogenic sequence changes in cancer cells by comparing cancer genome sequences against the draft genome. Apparently chimaeric transcripts (from oncogenic fusion genes generated by chromosomal translocations, the ends of which mapped to different genomic locations) were detected to the same degree in both normal and neoplastic tissues, indicating a significant level of false positives. Our experiment underscores the limited amount and variable quality of DNA sequence from cancer cells that is currently available.

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DO - 10.1038/35057046

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Cancer and genomics

Abstract

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