TY - JOUR
T1 - Cardiopulmonary Bypass and Host Defense Functions in Human Beings
T2 - II. Lymphocyte Function
AU - van Velzen-Blad, Heleen
AU - Dijkstra, Y. J.
AU - Heijnen, Cobi J.
AU - Schurink, G. A.
AU - Zegers, B. J.M.
AU - Ballieux, R. E.
N1 - Funding Information:
This work was supported by the Dutch Heart Foundation (Nederlandse Hartstichting, Grant No. 73119). Additional financial support was obtained from Travenol International Services, Inc., Brussels, Belgium. The authors are greatly indebted to Trui Matze for her excellent secretarial assistance.
PY - 1985
Y1 - 1985
N2 - In 47 patients undergoing open-heart surgical procedures, the influence of cardiopulmonary bypass (CPB) on lymphocyte function was investigated by studying in vitro the mitogen responses of lymphocytes in whole blood cultures. Subnormal mitogen responses before operation that likely resulted from dexamethasone medication were found in half of the patients studied. During operation, changes in phytohemagglutinin responses were uniform in a group of 23 patients. No significant effect of anesthesia and operation was observed until the patients were heparinized (i.e., before CPB). At the end of operation, the phytohemagglutinin response was below normal. In a group of 24 other patients, postoperative mitogen responses were studied. A tendency toward restoration of mitogen responses was observed in most patients the first morning after operation. However, no uniform pattern of normalization of mitogen responses was found. In an attempt to relate postoperative mitogen responses to trauma resulting from CPB, we observed that perioperative (in comparison with postoperative) administration of blood coincided with a significantly higher incidence of subnormal phytohemagglutinin and pokeweed mitogen responses on postoperative day 1. No correlation between laboratory data and clinical findings could be established.
AB - In 47 patients undergoing open-heart surgical procedures, the influence of cardiopulmonary bypass (CPB) on lymphocyte function was investigated by studying in vitro the mitogen responses of lymphocytes in whole blood cultures. Subnormal mitogen responses before operation that likely resulted from dexamethasone medication were found in half of the patients studied. During operation, changes in phytohemagglutinin responses were uniform in a group of 23 patients. No significant effect of anesthesia and operation was observed until the patients were heparinized (i.e., before CPB). At the end of operation, the phytohemagglutinin response was below normal. In a group of 24 other patients, postoperative mitogen responses were studied. A tendency toward restoration of mitogen responses was observed in most patients the first morning after operation. However, no uniform pattern of normalization of mitogen responses was found. In an attempt to relate postoperative mitogen responses to trauma resulting from CPB, we observed that perioperative (in comparison with postoperative) administration of blood coincided with a significantly higher incidence of subnormal phytohemagglutinin and pokeweed mitogen responses on postoperative day 1. No correlation between laboratory data and clinical findings could be established.
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U2 - 10.1016/S0003-4975(10)62581-7
DO - 10.1016/S0003-4975(10)62581-7
M3 - Article
C2 - 3977460
AN - SCOPUS:0021911263
SN - 0003-4975
VL - 39
SP - 212
EP - 217
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 3
ER -