TY - JOUR
T1 - Catalase HPI influences membrane permeability in Escherichia coli following near-UV stress
AU - Levén, Sara
AU - Heimberger, Amy
AU - Eisenstark, Abraham
N1 - Funding Information:
These investigations were supported by grants from the National Institutes of Health (ES04489), University of Missouri Institutional Research Support Grant RFR 3296 (N.I.H.), and by the Cancer Research Center of Columbia, Missouri. S.L. was an intern under the Howard Hughes Undergraduate Research Program.
PY - 1990/9/28
Y1 - 1990/9/28
N2 - The katG gene in Escherichia coli encodes catalase HPI, which is involved in membrane transport and protects the cell during oxidative stress. Hydrogen peroxide (H2O2) induces synthesis of HPI. We examined the role of HPI in membrane permeability (proline uptake) following exposure to near-ultraviolet radiation (NUV). We found that NUV resulted in the same type of induction as H2O2. KatG::Tn10 cells experienced a large drop in uptake after NUV exposure, and levels remained low following incubation. A strain carrying a katG+ plasmid, however, showed considerably less decrease in uptake after NUV, and uptake quickly resumed upon incubation. Further, in an srd mutant which lacks 4-thiouracil, NUV resulted in only a small drop in proline uptake, which was immediately resumed.
AB - The katG gene in Escherichia coli encodes catalase HPI, which is involved in membrane transport and protects the cell during oxidative stress. Hydrogen peroxide (H2O2) induces synthesis of HPI. We examined the role of HPI in membrane permeability (proline uptake) following exposure to near-ultraviolet radiation (NUV). We found that NUV resulted in the same type of induction as H2O2. KatG::Tn10 cells experienced a large drop in uptake after NUV exposure, and levels remained low following incubation. A strain carrying a katG+ plasmid, however, showed considerably less decrease in uptake after NUV, and uptake quickly resumed upon incubation. Further, in an srd mutant which lacks 4-thiouracil, NUV resulted in only a small drop in proline uptake, which was immediately resumed.
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U2 - 10.1016/0006-291X(90)90816-6
DO - 10.1016/0006-291X(90)90816-6
M3 - Article
C2 - 2222441
AN - SCOPUS:0025002704
SN - 0006-291X
VL - 171
SP - 1224
EP - 1228
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 3
ER -