TY - JOUR
T1 - Cell Counting Techniques for Preimplantation Mouse Embryos Using Fluorescent DNA Dyes
AU - Papaioannou, Virginia E.
AU - Behringer, Richard R.
N1 - Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press.
PY - 2023/12
Y1 - 2023/12
N2 - Counting cells in preimplantation embryos by light microscopy is straightforward until morula compaction, when cell boundaries in living embryos become indistinct. An alternative to morphological assessment of cell number is to use fluorescent DNA dyes. This protocol details simple nuclear counting with a single DNA dye (Hoechst) or a more complicated procedure in which differential nuclear counts of the trophectoderm and inner cell mass (ICM) can be made using immunosurgery of the blastocyst and two DNA dyes (Hoechst and propidium iodide).
AB - Counting cells in preimplantation embryos by light microscopy is straightforward until morula compaction, when cell boundaries in living embryos become indistinct. An alternative to morphological assessment of cell number is to use fluorescent DNA dyes. This protocol details simple nuclear counting with a single DNA dye (Hoechst) or a more complicated procedure in which differential nuclear counts of the trophectoderm and inner cell mass (ICM) can be made using immunosurgery of the blastocyst and two DNA dyes (Hoechst and propidium iodide).
UR - http://www.scopus.com/inward/record.url?scp=85182275347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85182275347&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot108091
DO - 10.1101/pdb.prot108091
M3 - Article
C2 - 37932075
AN - SCOPUS:85182275347
SN - 1940-3402
VL - 2023
SP - 975
EP - 978
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 12
ER -