TY - JOUR
T1 - Cell-Mediated Immunity Is Depressed Following Cardiopulmonary Bypass
AU - Roth, Jack A.
AU - Golub, Sidney H.
AU - Cukingnan, Ramon A.
AU - Brazier, John
AU - Morton, Donald L.
N1 - Funding Information:
Supported by Grant CA12582 awarded by the National Cancer Institute (DHHS) and by the Medical Research Service of the Veterans Administration. Accepted for publication July 7, 1980.
PY - 1981
Y1 - 1981
N2 - Sequential in vitro lymphocyte function tests in 13 patients undergoing cardiac operation were performed to determine factors that contribute to depressed cell-mediated immunity following operation. Lymphocytes were stimulated with phytohemagglutinin (PHA), pokeweed mitogen, concanavalin A (Con A), and mitomycin-treated, pooled, allogeneic lymphocytes (MLC). Mitogen responses were measured by3 H-labeled thymidine incorporation. Circulating levels of T, B, and Fc-receptor lymphocytes were determined by counting E, EAC, and EA rosettes. Serum cortisol was measured by radioimmunoassay. The T-cell-dependent lymphocyte responses (PHA, Con A, and MLC) were significantly decreased 24 hours after operation, and this was accompanied by a 60% decrease in circulating T-cell levels. The PHA, Con A, and MLC responses, and circulating T-cell levels returned to preoperative values one week following operation. Lymphocyte responses to mitogens remained significantly decreased when the number of T cells in the postoperative cultures were adjusted to preoperative levels. This indicates that the T cells remaining after operation were functionally impaired. We conclude that lymphocyte proliferative responses and antigen recognition are significantly depressed following cardiac operation, and that these responses are related to decreased numbers of circulating T lymphocytes and depressed function of the remaining T lymphocytes.
AB - Sequential in vitro lymphocyte function tests in 13 patients undergoing cardiac operation were performed to determine factors that contribute to depressed cell-mediated immunity following operation. Lymphocytes were stimulated with phytohemagglutinin (PHA), pokeweed mitogen, concanavalin A (Con A), and mitomycin-treated, pooled, allogeneic lymphocytes (MLC). Mitogen responses were measured by3 H-labeled thymidine incorporation. Circulating levels of T, B, and Fc-receptor lymphocytes were determined by counting E, EAC, and EA rosettes. Serum cortisol was measured by radioimmunoassay. The T-cell-dependent lymphocyte responses (PHA, Con A, and MLC) were significantly decreased 24 hours after operation, and this was accompanied by a 60% decrease in circulating T-cell levels. The PHA, Con A, and MLC responses, and circulating T-cell levels returned to preoperative values one week following operation. Lymphocyte responses to mitogens remained significantly decreased when the number of T cells in the postoperative cultures were adjusted to preoperative levels. This indicates that the T cells remaining after operation were functionally impaired. We conclude that lymphocyte proliferative responses and antigen recognition are significantly depressed following cardiac operation, and that these responses are related to decreased numbers of circulating T lymphocytes and depressed function of the remaining T lymphocytes.
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U2 - 10.1016/S0003-4975(10)60965-4
DO - 10.1016/S0003-4975(10)60965-4
M3 - Article
C2 - 7011230
AN - SCOPUS:0019426187
SN - 0003-4975
VL - 31
SP - 350
EP - 356
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 4
ER -