TY - JOUR
T1 - Cellular distribution of interleukin-1α-immunoreactivity after MPTP intoxication in mice
AU - Hébert, Guillaume
AU - Mingam, Rozenn
AU - Arsaut, Josette
AU - Dantzer, Robert
AU - Demotes-Mainard, Jacques
N1 - Funding Information:
This work was supported by INSERM, Université de Bordeaux-2 and Conseil Régional d'Aquitaine, and by France-Parkinson, which provided a fellowship grant to Guillaume Hébert.
PY - 2005/8/18
Y1 - 2005/8/18
N2 - In young rodents, peripheral injection of N-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) results in a dopaminergic nigrostriatal denervation (during the first week after injection), followed by a spontaneous dopaminergic reinnervation. Sprouting from residual neurons has been proposed to account for this event. It has been shown that an inflammatory process takes place during striatal dopaminergic denervation but its consequences remain controversial. Some clues notably indicate that interleukin (IL)-1α may participate in MPTP-induced inflammation and promote recovery. We therefore studied the immunohistochemical localization of IL-1α expression in the striatum and ventral mesencephalon at different times (1, 3, 6, 16, and 30 days) after MPTP injection in mice. IL-1α-immunoreactivity (ir) was observed in striatum, substantia nigra pars compacta, and ventral tegmental area. Apart from a few localization in mesencephalic activated microglia, IL-1α was almost exclusively found in activated astrocytes. However, in the striatal parenchyma, another component of IL-1α-ir colocalized with tyrosine hydroxylase (TH)-ir, a marker for dopaminergic neurons. Moreover, some parenchymal TH-positive axons were also found to express the growth cone-associated protein (GAP)-43, a marker for axonal growth cones. In the striatum, IL-1α-ir was also detected in a non-astrocytic perivascular component, with a distribution similar to GAP-43-ir. IL-1α could thus directly or indirectly influence striatal reorganization after MPTP.
AB - In young rodents, peripheral injection of N-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) results in a dopaminergic nigrostriatal denervation (during the first week after injection), followed by a spontaneous dopaminergic reinnervation. Sprouting from residual neurons has been proposed to account for this event. It has been shown that an inflammatory process takes place during striatal dopaminergic denervation but its consequences remain controversial. Some clues notably indicate that interleukin (IL)-1α may participate in MPTP-induced inflammation and promote recovery. We therefore studied the immunohistochemical localization of IL-1α expression in the striatum and ventral mesencephalon at different times (1, 3, 6, 16, and 30 days) after MPTP injection in mice. IL-1α-immunoreactivity (ir) was observed in striatum, substantia nigra pars compacta, and ventral tegmental area. Apart from a few localization in mesencephalic activated microglia, IL-1α was almost exclusively found in activated astrocytes. However, in the striatal parenchyma, another component of IL-1α-ir colocalized with tyrosine hydroxylase (TH)-ir, a marker for dopaminergic neurons. Moreover, some parenchymal TH-positive axons were also found to express the growth cone-associated protein (GAP)-43, a marker for axonal growth cones. In the striatum, IL-1α-ir was also detected in a non-astrocytic perivascular component, with a distribution similar to GAP-43-ir. IL-1α could thus directly or indirectly influence striatal reorganization after MPTP.
KW - GAP-43
KW - MPTP
KW - Striatum
KW - Substantia nigra
KW - Tyrosine hydroxylase
KW - Ventral tegmental area
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U2 - 10.1016/j.molbrainres.2005.04.019
DO - 10.1016/j.molbrainres.2005.04.019
M3 - Article
C2 - 15922486
AN - SCOPUS:23744466228
SN - 0169-328X
VL - 138
SP - 156
EP - 163
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -