@inbook{51ea4d6bc0e54c8da3f7c3d146c594c8,
title = "Cellular experiments to study the inhibition of c-Myc/MAX heterodimerization",
abstract = "The c-Myc oncogene is a master regulator of cancer cell metabolism, which controls a variety of pathways, including cell proliferation, cell cycle, apoptosis, and epigenetics. Belonging to the bHLH family of transcription factors, c-Myc forms a heterodimeric complex with another bHLH family protein MAX. c-Myc deregulation is reported in most cancers. This heterodimeric complex is a potent transcription factor that controls the expression of the target gene by binding to the E-box sequence and thereby controlling cancer cell proliferation. c-Myc in isolation has a partially folded structure and cannot carry the transcription. However, its heterodimerization provides the ability to bind DNA and carry out the regulatory function. Therefore, heterodimerization of c-Myc and Max is of great interest for cancers, and it has always been considered a target for cancer therapy. This book chapter will present a detailed protocol of cellular experiments employed to validate the in vitro potency of c-Myc inhibitor candidates to search for a novel c-Myc-targeted neoplastic drug.",
keywords = "c-Myc, Drug-discovery, Inhibitors, MAX, Protein–protein interaction",
author = "Ashutosh Singh and Shilpa Sharma and Praveen Kumar and Neha Garg",
note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = jan,
doi = "10.1016/bs.mie.2022.07.009",
language = "English (US)",
isbn = "9780323992664",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "193--205",
editor = "Shukla, {Arun K.}",
booktitle = "Integrated Methods in Protein Biochemistry",
}