Characterization of chain breakage in DNA induced by bleomycin

M. Tien Kuo; Charles W. Haidle

doi:10.1016/0005-2787(74)90216-0

Characterization of chain breakage in DNA induced by bleomycin

M. Tien Kuo, Charles W. Haidle

Translational Molecular Pathology

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

Abstract

Both deoxyribose moieties and phosphodiester bonds of DNA are destroyed by the action of bleomycin. A malonic aldehyde-like compound is produced in the drug-treated DNA. Phosphodiester bond rupture results in the production of 30 % of hydroxyl groups and 70 % of phosporyl groups in the 5′-termini of bleomycintreated DNA. Very few or perhaps none of the 3′-termini are phosphorylated.

Original language	English (US)
Pages (from-to)	109-114
Number of pages	6
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	335
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(74)90216-0
State	Published - Jan 31 1974

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General Medicine

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title = "Characterization of chain breakage in DNA induced by bleomycin",

abstract = "Both deoxyribose moieties and phosphodiester bonds of DNA are destroyed by the action of bleomycin. A malonic aldehyde-like compound is produced in the drug-treated DNA. Phosphodiester bond rupture results in the production of 30 % of hydroxyl groups and 70 % of phosporyl groups in the 5′-termini of bleomycintreated DNA. Very few or perhaps none of the 3′-termini are phosphorylated.",

author = "Kuo, {M. Tien} and Haidle, {Charles W.}",

note = "Funding Information: We wish to acknowledge the contribution of exonuclease III from Dr J. Hurwitz, and the helpful assistance of Mr Joel Elliott, Ms Mary Ann Tjaden, Ms Alice Glover and Ms Emma Broussard. This research was supported in part by Grant G-441 from The Robert A. Welch Foundation and Grant CA 13246 from the United States Public Health Service.",

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T1 - Characterization of chain breakage in DNA induced by bleomycin

AU - Kuo, M. Tien

AU - Haidle, Charles W.

N1 - Funding Information: We wish to acknowledge the contribution of exonuclease III from Dr J. Hurwitz, and the helpful assistance of Mr Joel Elliott, Ms Mary Ann Tjaden, Ms Alice Glover and Ms Emma Broussard. This research was supported in part by Grant G-441 from The Robert A. Welch Foundation and Grant CA 13246 from the United States Public Health Service.

PY - 1974/1/31

Y1 - 1974/1/31

N2 - Both deoxyribose moieties and phosphodiester bonds of DNA are destroyed by the action of bleomycin. A malonic aldehyde-like compound is produced in the drug-treated DNA. Phosphodiester bond rupture results in the production of 30 % of hydroxyl groups and 70 % of phosporyl groups in the 5′-termini of bleomycintreated DNA. Very few or perhaps none of the 3′-termini are phosphorylated.

AB - Both deoxyribose moieties and phosphodiester bonds of DNA are destroyed by the action of bleomycin. A malonic aldehyde-like compound is produced in the drug-treated DNA. Phosphodiester bond rupture results in the production of 30 % of hydroxyl groups and 70 % of phosporyl groups in the 5′-termini of bleomycintreated DNA. Very few or perhaps none of the 3′-termini are phosphorylated.

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Characterization of chain breakage in DNA induced by bleomycin

Abstract

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