Abstract
Cytotoxic T lymphocyte (CTL) responses to Gag have been most frequently linked to control of viremia whereas CTL responses to Nef have direct relationship with viral load. IFN-γ ELISpot assay was used to screen CTL responses at single peptide level directed at HIV-1 subtype C Gag and Nef proteins in 30 antiretroviral therapy naive HIV-1 infected Indian individuals. PBMCs from 73.3% and 90% of the study population showed response to Gag and Nef antigens, respectively. The magnitude of Gag-specific CTL responses was inversely correlated with plasma viral load (r = -0.45, P = 0.001), whereas magnitude of Nef-specific responses was directly correlated (r = 0.115). Thirteen immunodominant regions (6 in Gag, 7 in Nef) were identified in the current study. The identification of Gag and Nef-specific responses across HIV-1 infected Indian population and targeting epitopes from multiple immunodominant regions may provide useful insight into the designing of new immunotherapy and vaccines.
Original language | English (US) |
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Pages (from-to) | 47-56 |
Number of pages | 10 |
Journal | Medical Microbiology and Immunology |
Volume | 198 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2009 |
Externally published | Yes |
Keywords
- CTL responses
- ELISpot
- Gag
- HIV-1 specific
- India
- Nef
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Microbiology (medical)