Characterization of initiation of angiogenesis in early stages of prostate adenocarcinoma development and progression in a transgenic murine model

Manal Gabril; Jim Xuan; Madeleine Moussa; Colin P.N. Dinney; Joseph L. Chin; Jonathan I. Izawa

doi:10.1016/j.urology.2004.07.034

Characterization of initiation of angiogenesis in early stages of prostate adenocarcinoma development and progression in a transgenic murine model

Manal Gabril, Jim Xuan, Madeleine Moussa, Colin P.N. Dinney, Joseph L. Chin, Jonathan I. Izawa

Urology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A novel prostate-specific protein of 94 amino acids (PSP94) promoter/enhancer-directed transgenic murine model of prostate cancer (CaP) was examined to determine the temporal relationship of angiogenesis with the development and progression of CaP. Immunohistochemistry and in situ hybridization were performed to determine the protein and mRNA expression of basic fibroblast growth factor, vascular endothelial growth factor, and microvessel formation at different stages of CaP development and progression. Differential expression of basic fibroblast growth factor and vascular endothelial growth factor protein was found with the development and progression of CaP (P <0.0001 and P <0.0001, respectively). Angiogenesis was identified in CaP. mRNA expression of basic fibroblast growth factor and vascular endothelial growth factor was present from high-grade prostatic intraepithelial neoplasia to poorly differentiated CaP. The PSP94 gene is conserved in humans and rodents; therefore, the PSP94 transgenic murine model of CaP provides a preclinical model to test such antiangiogenic or other chemopreventive therapies that may be predictive of human CaP.

Original language	English (US)
Pages (from-to)	1233-1237
Number of pages	5
Journal	Urology
Volume	64
Issue number	6
DOIs	https://doi.org/10.1016/j.urology.2004.07.034
State	Published - Dec 2004

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Urology

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10.1016/j.urology.2004.07.034

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@article{90735ce4c3624abe8b25b2a40ba9155e,

title = "Characterization of initiation of angiogenesis in early stages of prostate adenocarcinoma development and progression in a transgenic murine model",

abstract = "A novel prostate-specific protein of 94 amino acids (PSP94) promoter/enhancer-directed transgenic murine model of prostate cancer (CaP) was examined to determine the temporal relationship of angiogenesis with the development and progression of CaP. Immunohistochemistry and in situ hybridization were performed to determine the protein and mRNA expression of basic fibroblast growth factor, vascular endothelial growth factor, and microvessel formation at different stages of CaP development and progression. Differential expression of basic fibroblast growth factor and vascular endothelial growth factor protein was found with the development and progression of CaP (P <0.0001 and P <0.0001, respectively). Angiogenesis was identified in CaP. mRNA expression of basic fibroblast growth factor and vascular endothelial growth factor was present from high-grade prostatic intraepithelial neoplasia to poorly differentiated CaP. The PSP94 gene is conserved in humans and rodents; therefore, the PSP94 transgenic murine model of CaP provides a preclinical model to test such antiangiogenic or other chemopreventive therapies that may be predictive of human CaP.",

author = "Manal Gabril and Jim Xuan and Madeleine Moussa and Dinney, {Colin P.N.} and Chin, {Joseph L.} and Izawa, {Jonathan I.}",

note = "Funding Information: This study was supported in part by the Canadian Urological Association Research Scholarship R3255A01, the Northeastern Section of the American Urological Association Research Scholarship R-02-019, and the Lawson Health Research Institute Internal Research Fund IRF-026-02 (J. I. I.). ",

year = "2004",

month = dec,

doi = "10.1016/j.urology.2004.07.034",

language = "English (US)",

volume = "64",

pages = "1233--1237",

journal = "Urology",

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T1 - Characterization of initiation of angiogenesis in early stages of prostate adenocarcinoma development and progression in a transgenic murine model

AU - Gabril, Manal

AU - Xuan, Jim

AU - Moussa, Madeleine

AU - Dinney, Colin P.N.

AU - Chin, Joseph L.

AU - Izawa, Jonathan I.

N1 - Funding Information: This study was supported in part by the Canadian Urological Association Research Scholarship R3255A01, the Northeastern Section of the American Urological Association Research Scholarship R-02-019, and the Lawson Health Research Institute Internal Research Fund IRF-026-02 (J. I. I.).

PY - 2004/12

Y1 - 2004/12

N2 - A novel prostate-specific protein of 94 amino acids (PSP94) promoter/enhancer-directed transgenic murine model of prostate cancer (CaP) was examined to determine the temporal relationship of angiogenesis with the development and progression of CaP. Immunohistochemistry and in situ hybridization were performed to determine the protein and mRNA expression of basic fibroblast growth factor, vascular endothelial growth factor, and microvessel formation at different stages of CaP development and progression. Differential expression of basic fibroblast growth factor and vascular endothelial growth factor protein was found with the development and progression of CaP (P <0.0001 and P <0.0001, respectively). Angiogenesis was identified in CaP. mRNA expression of basic fibroblast growth factor and vascular endothelial growth factor was present from high-grade prostatic intraepithelial neoplasia to poorly differentiated CaP. The PSP94 gene is conserved in humans and rodents; therefore, the PSP94 transgenic murine model of CaP provides a preclinical model to test such antiangiogenic or other chemopreventive therapies that may be predictive of human CaP.

AB - A novel prostate-specific protein of 94 amino acids (PSP94) promoter/enhancer-directed transgenic murine model of prostate cancer (CaP) was examined to determine the temporal relationship of angiogenesis with the development and progression of CaP. Immunohistochemistry and in situ hybridization were performed to determine the protein and mRNA expression of basic fibroblast growth factor, vascular endothelial growth factor, and microvessel formation at different stages of CaP development and progression. Differential expression of basic fibroblast growth factor and vascular endothelial growth factor protein was found with the development and progression of CaP (P <0.0001 and P <0.0001, respectively). Angiogenesis was identified in CaP. mRNA expression of basic fibroblast growth factor and vascular endothelial growth factor was present from high-grade prostatic intraepithelial neoplasia to poorly differentiated CaP. The PSP94 gene is conserved in humans and rodents; therefore, the PSP94 transgenic murine model of CaP provides a preclinical model to test such antiangiogenic or other chemopreventive therapies that may be predictive of human CaP.

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Characterization of initiation of angiogenesis in early stages of prostate adenocarcinoma development and progression in a transgenic murine model

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