Abstract
Hydrogen sulfide (H2S) is a highly neurotoxic gas. It is the second most common cause of gas-induced deaths. Beyond mortality, surviving victims of acute exposure may suffer long-term neurological sequelae. There is a need to develop countermeasures against H2S poisoning. However, no translational animal model of H2S-induced neurological sequelae exists. Here, we describe a novel mouse model of H2S-induced neurotoxicity for translational research. In paradigm I, C57/BL6 mice were exposed to 765 ppm H2S for 40 min on day 1, followed by 15-min daily exposures for periods ranging from 1 to 6 days. In paradigm II, mice were exposed once to 1000 ppm H2S for 60 minutes. Mice were assessed for behavioral, neurochemical, biochemical, and histopathological changes. H2S intoxication caused seizures, dyspnea, respiratory depression, knockdowns, and death. H2S-exposed mice showed significant impairment in locomotor and coordinated motor movement activity compared with controls. Histopathology revealed neurodegenerative lesions in the collicular, thalamic, and cortical brain regions. H2S significantly increased dopamine and serotonin concentration in several brain regions and caused time-dependent decreases in GABA and glutamate concentrations. Furthermore, H2S significantly suppressed cytochrome c oxidase activity and caused significant loss in body weight. Overall, male mice were more sensitive than females. This novel translational mouse model of H2S-induced neurotoxicity is reliable, reproducible, and recapitulates acute H2S poisoning in humans.
Original language | English (US) |
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Pages (from-to) | 46-64 |
Number of pages | 19 |
Journal | Annals of the New York Academy of Sciences |
Volume | 1400 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2017 |
Externally published | Yes |
Keywords
- acute toxicity
- hydrogen sulfide
- inhalation exposure
- neurodegeneration
- neurotoxicity
- translational model
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- History and Philosophy of Science