Khimiko-fermentativnyǐ sintez geneticheskikh élementov dlia ékspressii iskusstvennykh genov v kletkakh Bacillus subtilis.

I. A. Gorbunov; N. K. Daniliuk; A. A. Il'ichev; V. N. Krasnykh; A. I. Lomakin

Khimiko-fermentativnyǐ sintez geneticheskikh élementov dlia ékspressii iskusstvennykh genov v kletkakh Bacillus subtilis.

Translated title of the contribution: Chemico-enzymatic synthesis of genetic elements for expression of synthetic genes in Bacillus subtilis cells

I. A. Gorbunov, N. K. Daniliuk, A. A. Il'ichev, V. N. Krasnykh, A. I. Lomakin

Cancer Systems Imaging

Research output: Contribution to journal › Article › peer-review

Abstract

In order to obtain the recombinant Bacillus subtilis strain, a transcriptional-translational control unit of the alpha-amylase gene of B. amyloliquefaciens was synthesized. The oligodeoxyribonucleotides were prepared by the modified triester method in solution and by the solid-phase approach. Then these oligonucleotides were joined by DNA ligase into two fragments which were cloned in the phage M13mp9 DNA and the plasmid pBR327. A plasmid harboring the site regulating the transcription of the alpha-amylase gene may be employed as vector for cloning the promoter-containing fragments in E. coli cells.

Translated title of the contribution	Chemico-enzymatic synthesis of genetic elements for expression of synthetic genes in Bacillus subtilis cells
Original language	Russian
Pages (from-to)	647-654
Number of pages	8
Journal	Bioorganicheskaia khimiia
Volume	12
Issue number	5
State	Published - May 1986

ASJC Scopus subject areas

General Medicine

Cite this

@article{27339567076b40f589977cb6bd2efafb,

title = "Khimiko-fermentativnyǐ sintez geneticheskikh {\'e}lementov dlia {\'e}kspressii iskusstvennykh genov v kletkakh Bacillus subtilis.",

abstract = "In order to obtain the recombinant Bacillus subtilis strain, a transcriptional-translational control unit of the alpha-amylase gene of B. amyloliquefaciens was synthesized. The oligodeoxyribonucleotides were prepared by the modified triester method in solution and by the solid-phase approach. Then these oligonucleotides were joined by DNA ligase into two fragments which were cloned in the phage M13mp9 DNA and the plasmid pBR327. A plasmid harboring the site regulating the transcription of the alpha-amylase gene may be employed as vector for cloning the promoter-containing fragments in E. coli cells.",

author = "Gorbunov, {I. A.} and Daniliuk, {N. K.} and Il'ichev, {A. A.} and Krasnykh, {V. N.} and Lomakin, {A. I.}",

year = "1986",

month = may,

language = "Russian",

volume = "12",

pages = "647--654",

journal = "Bioorganicheskaia khimiia",

issn = "0132-3423",

publisher = "Izdatel'stvo Nauka",

number = "5",

}

TY - JOUR

T1 - Khimiko-fermentativnyǐ sintez geneticheskikh élementov dlia ékspressii iskusstvennykh genov v kletkakh Bacillus subtilis.

AU - Gorbunov, I. A.

AU - Daniliuk, N. K.

AU - Il'ichev, A. A.

AU - Krasnykh, V. N.

AU - Lomakin, A. I.

PY - 1986/5

Y1 - 1986/5

N2 - In order to obtain the recombinant Bacillus subtilis strain, a transcriptional-translational control unit of the alpha-amylase gene of B. amyloliquefaciens was synthesized. The oligodeoxyribonucleotides were prepared by the modified triester method in solution and by the solid-phase approach. Then these oligonucleotides were joined by DNA ligase into two fragments which were cloned in the phage M13mp9 DNA and the plasmid pBR327. A plasmid harboring the site regulating the transcription of the alpha-amylase gene may be employed as vector for cloning the promoter-containing fragments in E. coli cells.

AB - In order to obtain the recombinant Bacillus subtilis strain, a transcriptional-translational control unit of the alpha-amylase gene of B. amyloliquefaciens was synthesized. The oligodeoxyribonucleotides were prepared by the modified triester method in solution and by the solid-phase approach. Then these oligonucleotides were joined by DNA ligase into two fragments which were cloned in the phage M13mp9 DNA and the plasmid pBR327. A plasmid harboring the site regulating the transcription of the alpha-amylase gene may be employed as vector for cloning the promoter-containing fragments in E. coli cells.

UR - http://www.scopus.com/inward/record.url?scp=0022715724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022715724&partnerID=8YFLogxK

M3 - Article

C2 - 3089232

AN - SCOPUS:0022715724

SN - 0132-3423

VL - 12

SP - 647

EP - 654

JO - Bioorganicheskaia khimiia

JF - Bioorganicheskaia khimiia

IS - 5

ER -

Khimiko-fermentativnyǐ sintez geneticheskikh élementov dlia ékspressii iskusstvennykh genov v kletkakh Bacillus subtilis.

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this