Abstract
Many studies focused on the discovery of novel biomarkers for the diagnosis and treatment of disease states are facilitated by mass spectrometry-based technology. HPLC coupled to mass spectrometry is widely used; miniaturization of this technique using nano-liquid chromatography (LC)-mass spectrometry (MS) usually results in better sensitivity, but is associated with limited repeatability. The recent introduction of chip-based technology has significantly improved the stability of nano-LC-MS, but no substantial studies to verify this have been performed. To evaluate the temporal repeatability of chip-based nano-LCMS analyses, N-glycans released from a serum sample were repeatedly analyzed using nLC-PGC-chip-TOF-MS on three non-consecutive days. With an average inter-day coefficient of variation of 4 %, determined on log10- transformed integrals, the repeatability of the system is very high. Overall, chip-based nano-LC-MS appears to be a highly stable technology, which is suitable for the profiling of large numbers of clinical samples for biomarker discovery.
Original language | English (US) |
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Pages (from-to) | 4953-4958 |
Number of pages | 6 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 405 |
Issue number | 14 |
DOIs | |
State | Published - May 2013 |
Keywords
- Biomarker discovery
- N-Glycans
- Nano-LC-MS
- Time of flight
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry